肾透明细胞癌患者SALL4、Bmi-1蛋白水平及其与上皮-间质转化的关系OA
Levels of SALL4 and Bmi-1 protein in patients with renal clear cell carcinoma and their relationship with epithelial-mesenchymal transition
目的 探讨肾透明细胞癌患者婆罗双树样基因4(SALL4)、B细胞特异性莫洛尼氏白血病毒插入位点1(Bmi-1)蛋白水平及其与上皮-间质转化(EMT)的关系.方法 选取2020年3月至2024年3月该院肿瘤内科收治的手术治疗后的87例肾透明细胞癌患者作为研究对象.采用免疫组织化学法(简称免疫组化)检测患者癌组织及癌旁组织中EMT标志物(E-钙黏蛋白、N-钙黏蛋白、波形蛋白)蛋白表达情况,并计算染色评分.采用蛋白质免疫印迹法检测患者癌组织及癌旁组织中SALL4、Bmi-1蛋白及EMT标志物蛋白水平.依据所有患者癌组织中SALL4、Bmi-1蛋白水平的均值,将患者分为高SALL4水平组(≥均值)与低SALL4水平组(<均值)、高Bmi-1水平组(≥均值)与低Bmi-1水平组(<均值).采用Pearson相关分析肾透明细胞癌患者癌组织中E-钙黏蛋白、N-钙黏蛋白、波形蛋白与SALL4、Bmi-1蛋白水平的相关性.采用受试者工作特征(ROC)曲线分析癌组织中SALL4、Bmi-4蛋白对肾透明细胞癌患者发生EMT的预测价值.比较不同SALL4、Bmi-4水平患者E-钙黏蛋白、N-钙黏蛋白、波形蛋白水平的差异.结果 免疫组化检测结果显示,患者癌组织中E-钙黏蛋白免疫组化染色评分、蛋白表达阳性率均低于癌旁组织(P<0.001),N-钙黏蛋白、波形蛋白免疫组化染色评分及蛋白表达阳性率均高于癌旁组织(P<0.001).蛋白质免疫印迹法检测结果显示,患者癌组织中E-钙黏蛋白水平低于癌旁组织(P<0.001),N-钙黏蛋白、波形蛋白、SALL4蛋白、Bmi-1蛋白水平均高于癌旁组织(P<0.001).Pearson相关分析结果显示,肾透明细胞癌患者癌组织中SALL4、Bmi-1蛋白水平与E-钙黏蛋白水平均呈负相关(r=-0.656、-0.654,P<0.001),与N-钙黏蛋白、波形蛋白水平均呈正相关(r=0.636、0.635、0.668、0.674,P<0.001).高SALL4水平组癌组织中E-钙黏蛋白免疫组化染色评分及蛋白水平均低于低SALL4水平组,N-钙黏蛋白、波形蛋白免疫组化染色评分及蛋白水平均高于低SALL4水平组,差异均有统计学意义(P<0.05);高Bmi-1水平组癌组织中E-钙黏蛋白免疫组化染色评分及蛋白水平均低于低Bmi-1水平组,N-钙黏蛋白、波形蛋白免疫组化染色评分及蛋白水平均高于低Bmi-1水平组,差异均有统计学意义(P<0.05).发生EMT组癌组织SALL4、Bmi-1蛋白水平均高于未发生EMT组,差异均有统计学意义(P<0.05).ROC曲线分析结果显示,癌组织中SALL4、Bmi-1蛋白联合预测肾透明细胞癌患者发生EMT的曲线下面积(AUC)为0.849,大于二者单独预测的AUC(0.700、0.724),差异均有统计学意义(Z=3.121、3.014,P<0.05).结论 肾透明细胞癌患者癌组织中SALL4、Bmi-1蛋白水平均明显升高,且与EMT的发生均有关,提示SALL4、Bmi-1蛋白可能是肾透明细胞癌进展的重要靶点.
Objective To investigate the levels of sala-like gene 4(SALL4)and B cell-specific moloney leu-kemia virus insertion site 1(Bmi-1)protein in patients with renal clear cell carcinoma and their relationship with epithelial-mesenchymal transition(EMT).Methods A total of 87 patients with renal clear cell carcino-ma after surgical treatment admitted to the Department of Oncology of the hospital from March 2020 to March 2024 were selected as the research objects.The protein expression of EMT markers(E-cadherin,N-cad-herin,vimentin)in cancer tissues and adjacent tissues of patients was detected by immunohistochemistry(re-ferred to as immunohistochemistry),and the staining score was calculated.The protein levels of SALL4,Bmi-1 and EMT markers in cancer tissues and adjacent tissues were detected by Western blotting.According to the mean value of SALL4 and Bmi-1 protein levels in cancer tissues of all patients,the patients were divided into high SALL4 level group(≥mean value)and low SALL4 level group(<mean value),high Bmi-1 level group(≥mean value)and low Bmi-1 level group(<mean value).Pearson correlation was used to analyze the corre-lation between E-cadherin,N-cadherin,vimentin and SALL4,Bmi-1 protein levels in cancer tissues of patients with renal clear cell carcinoma.Receiver operating characteristic(ROC)curve was used to analyze the predic-tive value of SALL4 and Bmi-4 protein in cancer tissues for EMT in patients with renal clear cell carcinoma.The differences of E-cadherin,N-cadherin and vimentin levels in patients with different SALL4 and Bmi-4 lev-els were compared.Results The results of immunohistochemical detection showed that the immunohisto-chemical staining score and positive rate of protein expression of E-cadherin in cancer tissues were lower than those in adjacent tissues(P<0.001),and the immunohistochemical staining score and positive rate of protein expression of N-cadherin and vimentin were higher than those in adjacent tissues(P<0.001).The results of Western blotting showed that the level of E-cadherin protein in cancer tissues was lower than that in adjacent tissues(P<0.001),and the levels of N-cadherin,vimentin,SALL4 protein and Bmi-1 protein were higher than those in adjacent tissues(P<0.001).Pearson correlation analysis showed that the protein levels of SALL4 and Bmi-1 in renal clear cell carcinoma tissues were negatively correlated with the levels of E-cadherin(r=-0.656,-0.654,P<0.001),and positively correlated with the levels of N-cadherin protein and vimen-tin(r=0.636,0.635,0.668,0.674,P<0.001).The immunohistochemical staining scores and levels of E-cad-herin in cancer tissues of high SALL4 level group were lower than those of low SALL4 level group,and the immunohistochemical staining scores and protein levels of N-cadherin and vimentin in cancer tissues of high SALL4 level group were higher than those of low SALL4 level group,and the differences were statistically significant(P<0.05).The immunohistochemical staining score and protein level of cancer tissue protein in the high Bmi-1 level group were lower than those in the low Bmi-1 level group,and the immunohistochemical staining score and protein level of N-cadherin and vimentin were higher than those in the low Bmi-1 level group,and the differences were statistically significant(P<0.05).The protein levels of SALL4 and Bmi-1 from cancer tissue in the EMT group were higher than those in the non-EMT group,and the differences were statistically significant(P<0.05).The results of ROC curve analysis showed that the area under the curve(AUC)of SALL4 and Bmi-1 protein combined in cancer tissues to predict EMT in patients with renal clear cell carcinoma was 0.849,which was greater than the AUC of SALL4 and Bmi-1 protein predicted alone(0.700,0.724),and the differences were statistically significant(Z=3.121,3.014,P<0.05).Conclusion The pro-tein levels of SALL4 and Bmi-1 in renal clear cell carcinoma tissues are significantly increased,and they are re-lated to the occurrence of EMT,suggesting that SALL4 and Bmi-1 protein may be important targets for the progression of renal clear cell carcinoma.
陈敏;许丹妮;吴仁正;夏威夷
海南医科大学第一附属医院检验科,海南 海口 570102海南医科大学第一附属医院肿瘤内科,海南 海口 570102海南医科大学第一附属医院检验科,海南 海口 570102海南医科大学第一附属医院检验科,海南 海口 570102
医药卫生
肾透明细胞癌婆罗双树样基因4B细胞特异性莫洛尼氏白血病毒插入位点1上皮-间质转化
renal clear cell carcinomasala-like gene 4B-cell-specific moloney leukemia virus inser-tion site 1epithelial-mesenchymal transition
《检验医学与临床》 2026 (1)
13-19,7
海南省卫生健康行业科研项目(19A200504)海南医科大学学术提升支撑计划(XSTS2025112).
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