超声参数联合血清miR-129-5p、miR-654-5p对乳腺癌的诊断价值OA
Diagnostic value of ultrasound combined with serum miR-129-5p and miR-654-5p in breast cancer
目的 探讨超声参数联合血清miR-129-5p、miR-654-5p对乳腺癌的诊断价值.方法 选取2021年12月至2023年12月在湖北省十堰市太和医院住院进行手术治疗的93例女性乳腺癌患者作为乳腺癌组,同期收治的93例女性乳腺良性病变患者作为良性组,另选取同期在湖北省十堰市太和医院体检的93例女性健康体检者作为对照组.所有研究对象均进行超声检查,并记录相关参数[最大血流速度(Vmax)、血流阻力指数(RI)、搏动指数(PI)];采用实时荧光定量反转录聚合酶链反应检测所有研究对象血清miR-129-5p、miR-654-5p水平;绘制受试者工作特征(ROC)曲线分析Vmax、RI、PI及血清 miR-129-5p、miR-654-5p对乳腺癌的诊断价值.结果 良性组、乳腺癌组血清miR-129-5p、miR-654-5p水平均明显低于对照组,且乳腺癌组血清miR-129-5p、miR-654-5p水平均低于良性组,差异均有统计学意义(P<0.05).良性组、乳腺癌组Vmax、RI、PI均明显高于对照组,且乳腺癌组Vmax、RI、PI均高于良性组,差异均有统计学意义(P<0.05).TNM分期为Ⅲ～Ⅳ期、有淋巴结转移、中低分化程度的乳腺癌患者血清miR-129-5p、miR-654-5p低表达比例均高于TNM分期为Ⅰ～Ⅱ期、无淋巴结转移、高分化程度的乳腺癌患者,差异均有统计学意义(P<0.05).ROC曲线分析结果显示,Vmax、RI、PI、miR-129-5p、miR-654-5p联合诊断乳腺癌的曲线下面积(AUC)为0.892,大于5项单独诊断的AUC(0.712、0.783、0.720、0.648、0.718),差异均有统计学意义(Z=4.013、4.215、3.889、6.223、3.887,P<0.05).结论 乳腺癌患者血清miR-129-5p、miR-654-5p水平均降低,超声参数(Vmax、RI、PI)均升高,超声参数联合血清miR-129-5p、miR-654-5p诊断乳腺癌的价值较高.
Objective To investigate the diagnostic value of ultrasound combined with serum miR-129-5p and miR-654-5p in breast cancer.Methods A total of 93 female patients with breast cancer who underwent surgical treatment in Shiyan Taihe Hospital of Hubei Province from December 2021 to December 2023 were selected as the breast cancer group,93 female patients with benign breast lesions were selected as the benign group,and 93 female healthy subjects who underwent physical examination in Shiyan Taihe Hospital of Hubei Province during the same period were selected as the control group.All subjects underwent ultrasound exami-nation,and the related parameters[maximum blood flow velocity(Vmax),resistance index(RI),pulsatility index(PI)]were recorded.The levels of serum miR-129-5p and miR-654-5p in all subjects were detected by real-time fluorescent quantitative reverse transcription polymerase chain reaction.The receiver operating char-acteristic(ROC)curve was drawn to analyze the diagnostic value of Vmax,RI,PI combined with serum miR-129-5p and miR-654-5p in breast cancer.Results The serum levels of miR-129-5p and miR-654-5p in the be-nign group and the breast cancer group were significantly lower than those in the control group,and the serum levels of miR-129-5p and miR-654-5p in the breast cancer group were lower than those in the benign group,and the differences were statistically significant(P<0.05).The Vmax,RI and PI of the benign group and the breast cancer group were significantly higher than those of the control group,and the Vmax,RI and PI of the breast cancer group were higher than those of the benign group,and the differences were statistically signifi-cant(P<0.05).The low expression rates of serum miR-129-5p and miR-654-5p in breast cancer patients with TNM stage Ⅲ-Ⅳ,lymph node metastasis and moderate and low differentiation were higher than those in breast cancer patients with TNM stage Ⅰ-Ⅱ,no lymph node metastasis and high differentiation,and the differences were statistically significant(P<0.05).ROC curve analysis showed that the area under the curve(AUC)of the combination of Vmax,RI,PI,miR-129-5p and miR-654-5p in the diagnosis of breast cancer was 0.892,which was greater than the AUC of the five alone(0.712,0.783,0.720,0.648,0.718),the differences were all statistically significant(Z=4.013,4.215,3.889,6.223,3.887,P<0.05).Conclusion The levels of serum miR-129-5p and miR-654-5p in breast cancer patients were decreased,and the ultrasound(Vmax,RI,PI)were increased.Ultrasound parameters combined with serum miR-129-5p and miR-654-5p have high diagnostic value for breast cancer.
代妮娜;张文君;张华
湖北省十堰市太和医院/湖北医药学院附属太和医院超声医学科,湖北 十堰 442000湖北省十堰市太和医院/湖北医药学院附属太和医院超声医学科,湖北 十堰 442000湖北省十堰市太和医院/湖北医药学院附属太和医院甲乳外科,湖北 十堰 442000
医药卫生
超声参数miR-129-5pmiR-654-5p乳腺癌诊断价值微小RNA
ultrasound parametermiR-129-5pmiR-654-5pbreast cancerdiagnostic valuemi-croRNA
《检验医学与临床》 2026 (1)
1-6,6
湖北省科技厅自然科学基金创新发展联合基金项目(2024AFD092).
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