显微切割的胶质母细胞瘤蛋白质组学OA

Objective To analyze differentially expressed proteins in glioblastoma(GBM)tissues and to provide po-tential clews for early diagnosis of glioblastoma.Methods A total of 27 formalin fixed paraffin embedded(FFPE)specimens of glioblastoma tumor tissues,glioblastoma paired para-neoplastic tissues and non-tumor brain tissues were collected.The tissue samples were obtained using laser capture microdissection(LCM),followed by liquid chromatography-mass spectrometry for quantitative proteomic analysis.The screened differentially expressed proteins were subjected to functional analysis and three of them were selected for immunohistochemical validation.Results A total of 6 205 proteins were identified in these tissue samples.Compared with the non-tumor group,142 proteins were significantly expressed abnormally in the paraneoplastic group and 1 273 proteins in the tumor group;Com-pared with the paraneoplastic group,743 proteins were significantly expressed abnormally in the tumor group.PTBP1,AK2 and SHMT2 were studied and immunohistochemically verified,the results showed that all three proteins were significantly up-regulated in the tumor group(P＜0.05)as compared with the non-tumor group and paraneoplastic group.Conclusions Significant proteomic differences between tumor,para-neoplastic and non-tumor tissues of glioblastoma provide important clues for in-depth study of early diagnosis of glioblastoma.