空气中PM2.5加重慢性阻塞性肺疾病模型小鼠肺损伤OA

Objective To investigate the exacerbating effects of fine particulate matter(PM2.5)on lung injury in a chronic obstructive pulmonary disease(COPD)mouse model,and to provide a reliable experimental basis for further research.Methods Mice were randomly divided into four groups:control group,COPD group induced by cigarette smoke and lipopolysaccharide(LPS),KP group mice induced by intranasal instillation of Klebsiella pneu-monia(KP),and PM2.5 group(COPD model)induced by intranasal instillation of PM2.5).Lung function test was used to check forced vital capacity(FVC),inspiratory capacity(IC),forced expiratory volume in 0.1 seconds(FEV0.1),FEV0.2,FEV0.1/FVC ratio and FEV0.2/FVC ratio.Arterial blood gas analysis was applied to measure partial pressure of oxygen(PaO2),partial pressure of carbon dioxide(PaCO2),pH,and oxygen satura-tion(SaO2).Lung histopathology was evaluated via hematoxylin-eosin(HE)staining microscopy.Results Com-pared with the control group,COPD,KP and PM2.5 groups showed significantly increased FVC,IC,FEV0.1,FEV0.2 and PaCO2(P＜0.05),along with significantly decreasedFEV0.1/FVC,FEV0.2/FVC,PaO2 and SaO2(P＜0.05).Histo-pathology microscopy showed typical inflammatory responses and alveolar structural damage.Compared with the COPD group,KP and PM2.5 groups exhibited further increases in FVC,FEV0.1,and FEV0.2(P＜0.05),reducedFEV0.1/FVC and FEV0.2/FVC ratios,elevated IC and PaCO2(PM2.5 group only,P＜0.05),and decreased SaO2(PM2.5 group on-ly,P＜0.05).Histopathology microscopy confirmed aggravated inflammatory responses and alveolar destruction in these groups.Conclusions PM2.5 exacerbates pulmonary dysfunction and lung injury in COPD mice.