枸橼酸他莫昔芬促进TGF-β1对Leydig细胞系R2C的增殖OA

Objective To investigate the effect of tamoxifen citrate(TCT)on Leydig cell line R2C treated by TGF-β1.Methods R2C cells were used as the research subjects.CCK-8 test was used to detect the effect of different concentration gradients of TCT on the proliferation activity of normal R2C cells to fix on the most effective concen-tration.TGF-β1 was incubated with R2C cells for modeling,and then TCT was added into the medium at a concen-tration that significantly promoted R2C cell proliferation.R2C cell proliferation activity,basal testosterone,estradiol and cell cycle were detected.Results TGF-β1 treatment with 4 ng/mL,5 ng/mL and 6 ng/mL TCT for 24,48,and 72 hours significantly promoted R2C cell proliferation(P＜0.05);ELISA showed that treatment with 5 ng/mL and 6 ng/mL TCT for 48 hours resulted in a decrease in basal level of estradiol(E2)(P＜0.05)and an increase in basal level of testosterone(T)(P＜0.05).Flow cytometry showed a significant increase cell counting in G1 phase in the 6 ng/mL TCT treatment group,and the cell cycle arrested in the G1 phase.Conclusions A certain dose of TCT can improve the negative hormone level changes caused by exogenous injury of Leydig cell line R2C and pro-mote its proliferation.