生物合成FeS纳米颗粒加速海洋硫酸盐还原菌Desulfovibrio bizertensis SY-1胞外电子传递研究OA北大核心
Study on the accelerating extracellular electron transfer in marine sulfate-reducing bacterium Desulfovibrio bizertensis SY-1 by biosynthesis of FeS
为揭示生物合成FeS(Bio-FeS)对海洋硫酸盐还原菌Desulfovibrio bizertensis SY-1 胞外电子传递(EET)的调控机制,对加入20 mg/L、40 mg/L、60 mg/L、80 mg/L及100 mg/L Bio-FeS的Desulfobacterales bizertensis SY-1 体系通过计时电流法(I-t)、电化学阻抗谱(EIS)、差分脉冲伏安法(DPV)及循环伏安法(CV)等方法进行分析测试.结果表明,加入Bio-FeS后生物电流明显增强,Bio-FeS促进了D.bizertensis SY-1细胞色素 c 的合成进而增强氧化还原活性,同时也增强了电极界面电荷存储能力,降低了体系内电荷转移电阻及溶液电阻,提高了D.bizertensis SY-1 的胞外电子传递效率.这一研究为海洋工程中微生物腐蚀防护及微生物燃料电池(MFCs)的电子介体设计提供了新思路.
In order to reveal the regulation mechanism of biosynthesized FeS(Bio-FeS)on the extracellular electron transfer(EET)of the marine sulfate-reducing bacterium Desulfovibrio bizertensis SY-1(D.bizertensis SY-1),the addition of 20 mg/L,40 mg/L,60 mg/L,80 mg/L,and 100 mg/L Bio-FeS to the D.bizertensis SY-1 system with 20 mg/L,40 mg/L,60 mg/L,80 mg/L and 100 mg/L of Bio-FeS was analyzed and tested by chronoamperometry(I-t),electrochemical impedance spectroscopy(EIS),differential pulse voltammetry(DPV)and cyclic voltammetry(CV).The results showed that the bioelectrical current was significantly enhanced by the addition of Bio-FeS,and Bio-FeS promoted the synthesis of D.bizertensis SY-1 cytochrome c and thus enhanced the redox activity,and also enhanced the electrode interfacial charge storage capacity,lowered the intra-system charge transfer resistance and solution resistance,and increased the EET efficiency of D.bizertensis SY-1.electron transfer efficiency.This study provides new ideas for microbial corrosion protection in marine engineering and the design of electron mediators for microbial fuel cells(MFCs).
杨金峰;王亚楠;段继周
中国科学院海洋研究所 海洋关键材料重点实验室,山东 青岛 266000||中国科学院大学,北京 100049中国科学院海洋研究所 海洋关键材料重点实验室,山东 青岛 266000中国科学院海洋研究所 海洋关键材料重点实验室,山东 青岛 266000
能源科技
硫酸盐还原菌胞外电子传递计时电流法脉冲伏安法循环伏安法
sulfate-reducing bacteriaextracellular electron transferI-tDPVCV
《海洋科学》 2025 (6)
37-47,11
国家自然科学基金(4207604442206126)National Natural Science Foundation of China,Nos.42076044,42206126
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