电子束辐照杀菌对藏药木棉花和肉豆蔻有效成分的影响OA

To evaluate the effects of high-energy electron beam irradiation on the quality attributes of two Tibetan medicinal materials(Gossampini flos and Myristicae semen),samples were irradiated at doses of 0,2,4,6,8,and 10 kGy.Comprehensive analyses were conducted to assess changes in microbial load,chromatic characteristics,contents of principal bioactive constituents,and high-performance liquid chromatography(HPLC)fingerprint profiles before and after irradiation.The results indicated that irradiation at 2 kGy significantly reduced microbial counts compared to the non-irradiated control.Specifically,the total aerobic bacterial count,as well as mold and yeast counts,in G.flos were reduced to 175 and 20 CFU·g-1,respectively.In M.semen,the corresponding values were 210 and 20 CFU·g-1.At doses ≥2 kGy,both materials complied with the microbial limit standards stipulated in the Pharmacopoeia of the People's Republic of China(2020 edition),Volume Ⅳ.Compared to before irradiation,no significant changes were observed in the color characteristics of the G.flos powder after irradiation.However,the color characteristics of the M.semen powder showed significant changes at a dose of 6 kGy.At irradiation doses of 6-10 kGy,the content of the main active ingredients in the G.flos significantly increased,while the content of the main active ingredients in the M.semen showed no significant change.The HPLC fingerprint of the G.flos at different doses showed a similarity of over 0.970 compared to the non-irradiated group,indicating that electron beam irradiation affected the consistency of the flower's composition.In contrast,the similarity of the M.semen fingerprint decreased to 0.880 at 6 kGy and 0.809 at 8 kGy,suggesting that irradiation doses of 6-8 kGy impact the consistency of the M.semen composition.In conclusion,the minimum effective irradiation dose for both G.flos and M.semen was established at 2 kGy.The maximum tolerated dose was determined to be 10 kGy for G.flos and 6 kGy for M.semen.These findings support the feasibility of electron beam irradiation as a sterilization method for these materials and offer a scientific basis for the development of irradiation-based quality control standards for Tibetan medicinal powders.