基于转录组学分析白菜型冬油菜BraIAA基因家族OA

Auxin/indole acetic acid(Aux/IAA)genes is an early auxin responsive gene family,plays an important role in plant resistance to low temperature stress during growth and development.To explore the phylogeny and expression pattern of the BraIAA gene family in winter rapeseed,the whole genome identification and expression analysis were carried out,including gene structure,evolutionary relationship,chromosome distribution,conserved domain and physical and chemical properties.The expression characteristics of different genes across various varieties under different low temperature treatments were analyzed by fluorescence quantitative technique.The results reveald 62 IAA gene family members(BraIAA)in winter rapeseed genome.Phylogenetic tree classified them into five subfamilies,with uneven distribution across 10 chromosomes.Conserved domain analysis showed that these genes were short in length and lack introns.Synteny analysis demonstrated collinearity between BraIAA genes and their counterparts on five Arabidopsis thaliana chromosomes,while the distribution of cis-acting elements was diverse.Subcellular localization prediction showed that most of the genes were localized in the nucleus,while a few were localized in the endoplasmic reticulum and plasma membrane.Quantitative real-time PCR(qRT-PCR)analysis showed that Bra032521.1 and Bra030419.1 exhibited significant upregulation in both varieties under low temperature stress,while Bra040122.1 displayed a transient increase followed by decline in both varieties.The expression levels of Bra011082.1,Bra030219.1 and Bra040398.1decreased after treatment,while Bra022164.1 and Bra005508.1 showed opposite expression trends between two varieties.It can be inferred that Bra032521.1 and Bra030419.1 may play a positive regulatory role in low temperature stress response of winter rapeseed.This study provides a reference for exploring the function and regulation mechanism of the BraIAA gene family in winter rapeseed under low temperature stress.