水稻抗咪唑啉酮类除草剂基因标记开发及应用OA

The combination of herbicide resistant varieties and corresponding herbicides is an important method to control weed damage in rice fields.Marker-assisted selection can accelerate the breeding process of herbicide resistant varieties.To rapidly improve the herbicide resistance of rice varieties,a functional marker for the herbicide resistance gene was developed and rice variety HR1 with imidazolinone herbicides resistance was utilized to improve Yanghangnuo 1 in this study.Sequence alignment showed that the 548th encoded amino acid of ALS gene in the herbicide resistant variety was mutated from tryptophan to methionine(W548M),conferring resistance to ALS-inhibiting herbicides.To accelerate the breeding process,a functional marker ALS1642 was developed based on the principle of tetra-primer ARMS-PCR technology according to ALS gene sequence specificity.This marker can accurately identify the genotype associated with the herbicide resistance in seedlings.Using this marker,we screened the genetic segregation population derived from the cross between Yanghangnuo 1 and HR1 for herbicide resistance gene,and obtained several new rice lines carrying the gene.The results indicate that ALS1642 can be widely applied for marker-assisted selection of the W548M mutant genotype and the development of new rice varieties resistant to imidazolinone herbicides.This study is of great significance in improving herbicide resistance of rice varieties and enhancing breeding efficiency.