基于秀丽隐杆线虫模型的林下山参抗衰老作用研究OA

Objective To evaluate the anti-aging effects of mountain-cultivated ginseng using the Caenorhabditis elegans model and preliminarily investigate its mechanisms through network pharmacology analysis and RT-qPCR experiments.Methods The study employed Caenorhabditis elegans as an aging model organism.The impact of the water extract of mountain-cultivated ginseng on the thermotolerance of N2 wild-type Caenorhabditis elegans was observed using a heat stress challenge assay.Aging-related physiologi-cal indicators were measured,including lifespan,body length,locomotor ability,superoxide dismutase(SOD)and malondialdehyde(MDA)levels,lipofuscin accumulation,and reactive oxygen species(ROS)levels.Gene Ontology functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were performed based on network pharmacology.The"moun-tain-cultivated ginseng-chemical components-intersection targets-aging-signaling pathways"network was construct,followed by prelimi-nary validation of potential signaling pathways via uantitative real-time polymerase chain reaction(RT-qPCR).Results The water ex-tract of mountain-cultivated Ginseng significantly extended Caenorhabditis elegans lifespan,increased survival rates under heat stress,enhanced body length and locomotor activity,elevated SOD levels,and reduced accumulations of MDA,lipofuscin,and ROS,and the differences were statistically significant(P<0.05).Network pharmacological analysis indicates that biological processes exhibit a sig-nificant enrichment of targets;mountain-cultivated ginseng may exert its anti-aging pharmacological effects by modulating multiple sig-naling pathways,including PI3K-AKT,Ras,and HIF-1 signaling pathways.RT-qPCR results confirmed mountain-cultivated ginseng significantly downregulated the mRNA expression of age-1,akt-1,and daf-2,while markedly upregulating daf-16 mRNA expression levels(genes associated with the PI3K-AKT signaling pathway in Caenorhabditis elegans).Conclusion Mountain-cultivated ginseng effectively delays aging in Caenorhabditis elegans,primarily through suppression of the PI3K-AKT signaling pathway.