含BGN温敏粘接剂的制备及其对屏障膜的固定性能研究OA

Objective To investigate the barrier membrane fixation performance and enhanced guided bone regen-eration(GBR)capability of a thermosensitive adhesive containing bioactive glass nanoparticles in order to provide a novel solution for membrane fixation during GBR procedures.Methods M2NP@BGN(methoxyethyl acrylate-co-N-isopropylacrylamide-co-protocatechuic acid@Bioactive glass nanoparticle),a thermosensitive adhesive,was synthesized via free radical polymerization by compositing methoxyethyl acrylate,N-isopropylacrylamide,and protocatechuic acid into a basic adhesive that was modified with bioactive glass nanoparticle(BGN).The successful fabrication of basic ad-hesive M2NP was characterized by attenuated total reflection-Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy.The thermosensitive adhesive M2NP@BGN(BGN concentration of 1 mg/mL)was characterized by scanning electron microscopy and a rheometer.By adjusting the BGN concentration(0.1 mg/mL,0.5 mg/mL,1 mg/mL,and 2 mg/mL),the adhesive and mechanical strengths were investigated with a universal testing machine.Biocompatibility was evaluated with a cell counting kit-8 assay and hemolysis test to identify the optimal for-mulation.The optimal material's extract was co-cultured with mouse bone marrow mesenchymal stem cells,and its os-teogenic activity was examined in vitro by quantitative real-time PCR,alkaline phosphatase,and alizarin red S staining.The rat mandibular defect model was established,filled with bone graft,and divided into 3 groups based on membrane fixation method:M2NP@BGN(BGN concentration of 1 mg/mL)fixation group(M2NP@BGN),titanium nail fixation group(Nail),and unfixed control group(Negative).Bone regeneration was analyzed after 8 weeks by micro computed to-mography and histological staining.Results M2NP@BGN(BGN concentration of 1 mg/mL)was successfully synthe-sized and demonstrated rapid gelation under warm,humid conditions.The adhesive with a BGN concentration of 1 mg/mL exhibited the highest adhesive strength(P<0.001)and significantly enhanced mechanical strength(P<0.001)under 37℃ wet conditions.All formulations showed excellent biocompatibility,with cell viability>80%and hemolysis ratio<5%.M2NP@BGN(BGN concentration of 1 mg/mL)significantly upregulated the expression of Runx2 and Col I(P<0.001)and enhanced the activity of osteogenic differentiation markers(P<0.05).In the animal model,the M2NP@BGN group(BGN concentration of 1 mg/mL)achieved significantly higher bone volume fraction and better bone maturity compared to the negative and nail groups(P<0.05).Conclusion M2NP@BGN(BGN concentration of 1 mg/mL)combines excellent wet adhesion with potent osteogenic activity,enhances the bone augmentation efficacy of mem-branes,and presents a novel fixation strategy with significant clinical translation potential for GBR therapy.