兼具自矿化与成骨诱导特性的六磷酸肌醇-锌水凝胶的制备及性能评价OA

Objective To fabricate a hydrogel loaded with inositol hexaphosphate-zinc and preliminarily evaluate its performance in self-mineralization and osteoinduction,thereby providing a theoretical basis for the development of bone regeneration materials.Methods The hydrogel framework(designated DF0)was formed by copolymerizing meth-acryloyloxyethyltrimethylammonium chloride and four-armed poly(ethylene glycol)acrylate,followed by sequentially loading inositol hexaphosphate anions via electrostatic interaction and zinc ions via chelation.The hydrogel loaded only with inositol hexaphosphate anions was named DF1,while the co-loaded hydrogel was named DF2.The self-mineralization efficacy of the DF0,DF1 and DF2 hydrogels was characterized using scanning electron microscopy,trans-mission electron microscopy(TEM),energy dispersive spectroscopy(EDS),and selected area electron diffraction(SAED).The biocompatibility was assessed via live/dead cell staining and a CCK-8 assay.The osteoinductive capacity of the DF0,DF1 and DF2 hydrogels on MC3T3-E1 cells was assessed via alkaline phosphatase(ALP)and Alizarin Red S(ARS)staining.In the aforementioned cell experiments,cells cultured in standard medium served as the control group.Results The DF0,DF1,and DF2 hydrogels were successfully synthesized.Notably,DF1 and DF2 exhibited dis-tinct self-mineralization within 6 days.Results from TEM,EDS,and SAED confirmed that the mineralization products were amorphous calcium phosphate in group DF1,and amorphous calciumzinc phosphate in group DF2.Biocompatibility tests revealed that none of the hydrogels(DF0,DF1,and DF2)adversely affected cell viability or proliferation.In osteo-genic induction experiments,both ALP and ARS staining were intensified in the DF1 and DF2 groups,with the most pro-found staining observed in the DF2 group.Conclusion The developed inositol hexaphosphate-zinc hydrogel(DF2)dem-onstrates the dual capacity to generate calcium-phosphate compounds through self-mineralization while exhibiting excel-lent osteoinductive properties.This biocompatible,dual-promoting osteogenic hydrogel presents a novel strategy for bone regeneration.