液晶-水界面生物传感器的构建及其对食源性致病菌的可视化检测OA

The rapid and highly sensitive detection of foodborne pathogenic bacteria is crucial for food safety.In this study,by optimizing the modification conditions of silane reagent N,N-dimethyl-N-octadecyl-3-aminopropyltrimethoxysilyl chloride(DMOAP),cationic surfactant cetyltrimethylam-monium bromide(CTAB)and aptamers,a highly sensitive and visualizable liquid crystal-water inter-face sensor was successfully constructed.In this biosensor,CTAB was used to regulate the alignment of liquid crystal molecules,and aptamers were combined for the specific recognition of target patho-genic bacteria.The transformation of the ordered and disordered states of liquid crystal molecules was observed by polarizing microscopy,enabling the rapid detection of foodborne pathogenic bacteria(Salmonella typhimurium,Staphylococcus aureus and Escherichia coli).The detection limits reached 13,22 and 28 CFU/mL respectively,and the response time was shortened to 5 minutes.Specificity experiments could distinguish non-target bacteria without complex labeling or amplification steps.This technology provides a label-free and highly sensitive new solution for on-site screening of food-borne pathogenic bacteria,and has the potential to replace traditional time-consuming detection methods.