基于钒刺激的假单胞菌处理含钒氨氮废水研究OA
Purification of Vanadium-Containing Ammonia Nitrogen Wastewater by Pseudomonas sp.under Vanadium Stimulation
为推动钒工业绿色可持续发展,针对沉钒后氨氮废水污染环境、回用浸出工艺会降低钒浸出效率且导致管道阻塞的问题,采用假单胞菌处理含钒高浓度氨氮废水.结果显示:ρ(钒)≤100 mg·L-1 时,可促进假单胞菌生长,增大膜内外电势差与细胞膜通透性,增加胞外聚合物负电性官能团(如 C=O、N-H 等)含量,提升细菌物质运输及NH+4 吸附能力;ρ(钒)=100 mg·L-1时,氨氮去除率较无钒条件提高 6.64%.钒经假单胞菌胞外被动吸附与胞内主动累积进入菌体,作为酶系统刺激剂,使硝酸还原酶、氨单加氧酶的比酶活力分别增长 16.25%、123.11%,强化硝化过程.研究表明,钒通过"吸附累积-酶活性提升-底物运输强化"协同刺激假单胞菌酶系统,为高浓度氨氮废水深度净化提供潜在手段.
To promote green and sustainable development of the vanadium industry,this study employed Pseudomonas sp.to treat vanadium-containing high-concentration ammonia-nitrogen wastewater,aiming to address severe environmental pollution from post-vanadium-precipitation am-monia-nitrogen wastewater,as well as reduced vanadium leaching efficiency and pipeline blockage caused by wastewater reuse in leaching processes.Results showed that when ρ(vanadium)≤100 mg·L-1,it promoted Pseudomonas sp.growth,increased transmembrane potential difference and cell membrane permeability,enhanced negatively charged functional groups(e.g.,C=O,N-H)in extracellular polymeric substances(EPS),and improved bacterial substance transport and NH+4 adsorption capacities.At ρ(vanadium)=100 mg·L-1,the ammonia-nitrogen removal rate increased by 6.64%compared to the vanadium-free control.Vanadium entered bacterial cells through extracellular passive adsorption and intracellular active accumulation,acting as a stimulant to the enzyme system,significantly enhancing nitrification-related enzyme activities:nitrate reduc-tase specific activity increased by 16.25%and ammonia monooxygenase by 123.11%,thereby strengthening nitrification.This study reveals that vanadium synergistically stimulates Pseudomonas sp.enzyme system through"adsorption-accumulation-enzyme activity enhancement-substrate trans-port reinforcement",providing a potential approach for advanced purification of high-concentration ammonia-nitrogen wastewater.
佘依洹;张一敏;郑秋实;薛楠楠;蔡震雷
武汉科技大学资源与环境工程学院,湖北 武汉 430081||国家环境保护矿冶资源利用与污染控制重点实验室,湖北 武汉 430081||战略钒资源利用省部共建协同创新中心,湖北 武汉 430081武汉科技大学资源与环境工程学院,湖北 武汉 430081||国家环境保护矿冶资源利用与污染控制重点实验室,湖北 武汉 430081||战略钒资源利用省部共建协同创新中心,湖北 武汉 430081||湖北省页岩钒资源高效清洁利用工程技术研究中心,湖北 武汉 430081武汉科技大学资源与环境工程学院,湖北 武汉 430081||国家环境保护矿冶资源利用与污染控制重点实验室,湖北 武汉 430081||战略钒资源利用省部共建协同创新中心,湖北 武汉 430081武汉科技大学资源与环境工程学院,湖北 武汉 430081||国家环境保护矿冶资源利用与污染控制重点实验室,湖北 武汉 430081||战略钒资源利用省部共建协同创新中心,湖北 武汉 430081武汉科技大学资源与环境工程学院,湖北 武汉 430081||国家环境保护矿冶资源利用与污染控制重点实验室,湖北 武汉 430081||战略钒资源利用省部共建协同创新中心,湖北 武汉 430081
化学化工
含钒页岩含钒氨氮废水假单胞菌生物脱氮氨单加氧酶硝化反应
vanadium-bearing shalevanadium-containing ammonia nitrogen wastewaterPseudomonasbiological denitrificationammonia monooxygenasenitrification
《福建师范大学学报（自然科学版）》 2026 (1)
20-28,9
国家重点研发计划项目(2023YFC3903902)湖北省科技创新人才及服务专项(2022EJD002)
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