低温胁迫对黄脊竹蝗卵滞育的消除影响及其转录组分析OA

Ceracris kiangsu Tsai is a major pest seriously threatens forestry and grassland ecosystems,with its egg diapause being a critical factor for overwinter survival and population outbreaks.In this study we investi-gated the molecular response mechanisms of egg diapause release in C.kiangsu by applying different low tem-peratures(3℃and 8℃)to the diapausing eggs and combining with transcriptome sequencing.The results showed that the diapause release effect was closely related to the duration and temperature of low-temperature treatment,with a 60-day period being a critical turning point for shortened hatching and increased hatching rates.Hatching rates were significantly higher at 8℃compared to 3℃.Transcriptome analysis revealed that compared to the 25℃control group,3℃and 8℃treatments identified 9730 and 6075 differentially expressed genes(FDR<0.05,|log2FC|>1),respectively.GO and KEGG enrichment analyses revealed that these genes were significantly enriched in metabolic processes,including arginine metabolism,proline metabolism,and insect hormone biosynthesis,and stress responses,including FOXO signaling pathway and MAPK signal-ing pathway.Key genes such as ADAMTSL4,ALDH16A1,and foxo were identified,which regulate meta-bolic processes and developmental progression under low-temperature stress.These findings demonstrate novel insights into the molecular mechanisms of diapause release and highlight the potential for developing new green control targets for managing this pest.