电针不同留针时间对脑外伤昏迷大鼠前额叶皮质GABAB受体表达的影响OA

Objective:To observe the effects of different needle retention times of electroacupuncture on the expression of gamma-aminobutyric acid B(GABAB)receptors in the prefrontal cortex of rats with traumatic brain injury-induced coma,and to explore the potential neural mechanism of electroacupuncture in promoting arousal.Methods:Twenty-four clean-grade rats were randomly divided into a blank group,a model group,electroacupuncture group A,and electroacupuncture group B according to a random number table,with 6 rats in each group.The blank group received no treatment.Rats in the other groups were used to establish a traumatic brain injury-induced coma model using the free-fall impact method.After successful modeling,electroacupuncture group A and electroacupuncture group B received electroacupuncture stimulation at Baihui(GV20)and Shuigou(GV26)acupoints for 20 minutes and 30 minutes respectively,following needle insertion.The model group only received needle insertion,and the blank group received no treatment.The intervention was administered once daily for 7 consecutive days.Behavioral changes in the rats were observed.Prefrontal cortex tissue was harvested,and Western blot was used to detect the protein expression of GABAB,aquaporin-4(AQP-4),and malondialdehyde(MDA)in the prefrontal cortex.Results:Compared with the blank group,the protein expression levels of GABAB receptors,AQP-4,and MDA in the prefrontal cortex of the model group were significantly increased(P<0.001).Compared with the model group,the protein expression levels of AQP-4 and MDA in electroacupuncture group A were significantly decreased(P<0.01);the protein expression levels of AQP-4 and MDA in electroacupuncture group B were extremely significantly decreased(P<0.001);the protein expression levels of GABAB receptors in both electroacupuncture group A and electroacupuncture group B were significantly decreased(P<0.01).Compared with electroacupuncture group A,electroacupuncture group B showed a decreasing trend in the protein expression of GABAB receptors,AQP-4,and MDA,but the difference was not statistically significant.Conclusion:The GABAB receptor is an important neurotransmitter involved in the arousal-promoting effect of electroacupuncture.The molecular mechanism of electroacupuncture promoting arousal may be related to the down-regulation of GABAB receptor expression.No additional effect on the down-regulation of GABAB receptor expression was observed with prolonged electroacupuncture needle retention time.