毛相思子黄酮类生物合成关键酶基因的筛选及鉴定OA北大核心

Objective:To screen and identify the key enzyme genes in the flavonoid component synthesis pathway of Abrus mollis,so as to provide a necessary theoretical basis for the full utilization of the medicinal resources of Abrus mollis.Methods:Metabolomics was used to analyze and identify the differential components of flavonoids in the leaves and stems of Abrus mollis.Pathway enrichment a-nalysis was conducted using transcriptome sequencing and bioinformatics databases to screen out the key genes related to flavonoid bio-synthesis.Results:Through metabolomics analysis,a total of 196 flavonoids with different contents were obtained in the leaves and stems of Abrus mollis.Among them,the concent of 47 compounds in the stems were higher than those in the leaves,and 149 compounds con-tents in the leaves were higher than those in the stems.The contents of active components such as calycosin,luteolin,formononetin in the stems and leaves were significantly different.Transcriptome analysis indicated that in the comparison groups of different parts,13 syn-thetic enzymes such as PAL,4CL,C4H,HID jointly regulated 32 flavonoid components.The results of qRT-PCR showed that the expres-sion trends of the 8 randomly selected enzyme genes involved in flavonoid synthesis in the leaves and stems of Abrus mollis were consist-ent with the results of transcriptome sequencing.Conclusion:There are significant differences in the contents of flavonoid components such as calycosin in the stems and leaves of Abrus mollis.The differential expression of key enzyme genes in their synthetic pathways has a significant regulatory effect on the flavonoid components in Abrus mollis.The research results have important guiding significance for the rational utilization of different parts of Abrus mollis in the future.