铁皮石斛种苗工厂化生产工艺优化研究OA北大核心

Objective:To optimize the factory production process of Dendrobium officinale seedlings,in order to reduce the cost of seedling production and increase the survival rate of transplanting.Methods:Dendrobium officinale seeds were cultured in liquid suspen-sion for 30 days,and then 50 seeds were sucked up after the seeds turned into protocorms,visualized and sown into different formula-tions of medium,and the best medium was screened by the growth potential of test-tube seedlings after 120 days of cultivation;Coconut husk was used as a support instead of agar during the rooting stage,and the transplanting was carried out after 90 days of cultivation,the survival rate of transplanting,the number of sprouts,and the number of roots at 60 days after transplanting were statistically analyzed.Results:The highest induction rate of protocorms was observed after 30 days of N6 liquid suspension culture of Dendrobium officinale seeds.The germination density of test-tube seedlings was uniform after visualized and sown to 1/2 MS+5%potato juice+15%banana juice+0.35 mg/L NAA+30 g/L sucrose+7.5 g/L agar medium,and they could grow up to 3~4 cm without going through the stout transfer stage;The rooting status was good after transferring to MS+7.5%banana juice+7.5%banana juice+7.5%potato juice+30 g/L sucrose+2.0 mg/L 6-BA+0.5 mg/L NAA+3 cm bottled height coconut sugar:perlite 4∶1 per bottle,the rooting condition was good and the root system was green,and the survival rate of direct transplanting reached more than 96%without going through seedling refi-ning and seedling washing.Conclusion:The test results show that the use of seed liquid sprouting visualization precision sowing can e-liminate the strong seedling transfer stage of the current process,combined with the substrate alternative rooting technology,it can re-duce the seedling production cost of at least 30%or more,and has a good application and promotion value.