微量元素肥对北苍术生长、有效成分积累及其生物合成关键酶基因表达的影响OA北大核心

Objective:To study the effects of trace element fertilizers on the growth,aceumulation of active ingredients and the expression of key enzyme genes in the synthesis of Atractylodes chinensis,in order to provide a theoretical basis for the standardized cul-tivation of Atractylodes chinensis medicinal materials.Methods:A field experiment was conducted.The plant height,rood and stem diam-eter,fresh weight of stems and leaves and frest weight of rhizome of Atractylodes chinensis under different fertilization treatment were measured and recorded by the general determination method.The contents of four effective components(atractylenolideⅠ,β-eudesmol,atractylodin and atractylon)and the relative expression levels of three key enzyme genes(ACC,HMGR and FPPS)in Atractylodes chinensis were determined by high performance liquid chromatography and real-time fluorescence quantitative PCR.Results:Compared with ck,the plant height,rhizome diameter,stem and leaf fresh weight and rhizome fresh weight of Atractylodes chinensis treated with Y-2 and J-1 fertilization all increased significantly(P<0.05).Under the foliar spraying method of trace element fertilizer,the contents of atractylenolideⅠ,β-eudesmol,atractylodin and atractylon in the rhizome were significantly higher that ck treatment(P<0.05),and the contents of the four active ingredients in the Y-2 fertilization treatment were all the highest.Under the fertilizer based application of trace elements,the contents of atractylenolideⅠ,β-eudesmol,atractylodin and atractylon were significantly higher than CK treatment(P<0.05),and the contents of the four effective components were the highest under J-1 fertilization treatment.The contents of atractylodin and atractylon and the relative expression level of key enzyme gene HMGR under Y-2 fertilization treatment were the highest.The con-tents of atractylenolideⅠ,β-eudesmol and the relative expression level of key enzyme gene ACC under J-1 fertilization treatment were the highest.The correlation analysis showed that the polyacetylene component atractylodin had the highest correlation with the key en-zyme gene ACC(0.736,P<0.01).The sesquiterpene components atractylenolideⅠ and β-eudesmol had the extremely significant cor-relation with the key enzyme gene FPPS(P<0.01).The relative expression levels among the key enzyme genes ACC,FPPS and HMGR were all positively correlated. Conclusion:Foliar spraying scheme Y-2 (trace element application amount of 2. 6 g/7. 8 m2) is more conduciveto promoting the growth and accumulation of effective components of Atractylodes chinensis.