饱和氢生理盐水预防小鼠噪声性听力损失的实验研究OA
Protective effects of hydrogen-saturated saline on noise-induced hearing loss in mice
目的 探究饱和氢生理盐水预防噪声性听力损失的作用.方法 取SPF级、听性脑干反应阈值正常的8周龄C57BL/6J雄性小鼠24只,随机分成对照(WT)组、稳态噪声暴露(NG)组、饱和氢生理盐水预防(HS)组各8只.WT组给予生理盐水,1 mL/100g,每日腹腔注射,连续3 d,NG组于稳态噪声暴露前3 d每日腹腔注射等剂量生理盐水,HS组在接受稳态噪声暴露前3 d每日腹腔注射等剂量饱和氢生理盐水.于噪声暴露前、暴露后1、3、7 d行听性脑干反应测听.结束后处死动物,分离耳蜗中回基底膜以观察扫描电镜及免疫荧光染色.结果 NG组、HS组均出现听力损失,HS组各测听点听性脑干反应阈值均低于NG组,差异有统计学意义(P<0.05).HS组耳蜗外毛细胞纤毛形态受损程度轻于NG噪声组.HS组外毛细胞丢失数量少于NG噪声组,差异有统计学意义(P<0.05),HS组、NG组耳蜗基底膜带状突触数量均减少,NG组与HS组比较,差异无统计学意义(P>0.05).结论 噪声暴露前3 d,每日腹腔注射饱和氢生理盐水对由稳态噪声导致的小鼠听力损伤具有防护作用,对探索氢防护作用的分子机制具有一定的参考价值.
Objective The purpose of this study is to investigate potential roles of saturated hydrogen saline in prevention of noise-induced hearing loss(NIHL).Methods Twenty-four 8-week-old specific pathogen free(SPF)grade C57BL/6J male mice with normal auditory brainstem response(ABR)thresholds were randomly divided into normal control(wild type,WT),noise exposure(NG),and saturated hydrogen saline prophylaxis before noise exposure(HS)groups(n=8 in each group).The control group received intraperitoneal saline solution(1 mL/100 g/day)for three days.The NG group received the same saline treatment followed by noise exposure,whereas the HS group received an equivalent dose of saturated hydrogen saline before noise exposure.ARB audiometry was performed prior to and at 1,3 and 7 days after noise exposure.At the conclusion of the study,the middle cochlear turn basilar membrane was harvested and examined by scanning electron microscopy with immunofluorescence staining.Results Hearing loss was observed in both the NG and HS groups,with better ABR thresholds,less severe cochlear outer hair cells damage and less outer hair cells loss in the HS group than in the NG group(P<0.05).The degree of damage to the cilia morphology of cochlear outer hair cellsin the HS group was milder than that in the NG group.Cochlear basilar membrane ribbon synapses decreased in both HS and NG groups,and less in the NG group than in the HS group(P>0.05).Conclusions Prophylactic daily intraperitoneal injection of saturated hydrogen saline has a protective effect on hearing in mice against exposure to steady-state noise.This knowledge may have certain value in understanding molecular mechanisms of hydrogen protection.
TANG Jinge;REN Wei;WEI Cong;QI Guowei;SHI Lei;JIANG Qingqing;YUAN Shuolong;ZHU Yuhua;YU Ning
Clinical Medicine School of Chuanbei Medical College,Nanchong 637007,China||Department of Otorhinolaryngology Head and Neck Surgery,the Sixth Medical Center of the Chinese People's Liberation Army General Hospital,Beijing 100853,ChinaDepartment of Otorhinolaryngology Head and Neck Surgery,the Sixth Medical Center of the Chinese People's Liberation Army General Hospital,Beijing 100853,ChinaClinical Medicine School of Chuanbei Medical College,Nanchong 637007,China||Department of Otorhinolaryngology Head and Neck Surgery,the Sixth Medical Center of the Chinese People's Liberation Army General Hospital,Beijing 100853,ChinaDepartment of Otorhinolaryngology Head and Neck Surgery,the Sixth Medical Center of the Chinese People's Liberation Army General Hospital,Beijing 100853,ChinaDepartment of Otorhinolaryngology Head and Neck Surgery,the Sixth Medical Center of the Chinese People's Liberation Army General Hospital,Beijing 100853,ChinaDepartment of Otorhinolaryngology Head and Neck Surgery,the Sixth Medical Center of the Chinese People's Liberation Army General Hospital,Beijing 100853,ChinaDepartment of Otorhinolaryngology Head and Neck Surgery,the Sixth Medical Center of the Chinese People's Liberation Army General Hospital,Beijing 100853,ChinaDepartment of Otorhinolaryngology Head and Neck Surgery,the Sixth Medical Center of the Chinese People's Liberation Army General Hospital,Beijing 100853,ChinaDepartment of Otorhinolaryngology Head and Neck Surgery,the Sixth Medical Center of the Chinese People's Liberation Army General Hospital,Beijing 100853,China
稳态噪声噪声性听力损失饱和氢生理盐水
steady state noisenoise-induced hearing losssaturated hydrogen saline
《中华耳科学杂志》 2026 (1)
58-62,5
国家重点研发项目(2020YFC2004001)北京市自然科学基金项目(7222185)
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