青岛文昌鱼消化道真核生物的组成及季节差异OA

To investigate the dietary composition and seasonal variability of eukaryotes in wild populations of Bran-chiostoma japonicum,we employed 18S rDNA amplicon sequencing was employed to assess the community structure and seasonal fluctuations of eukaryotes within the digestive tract of B.japonicum from natural waters in Qingdao.The findings revealed a diverse community within the digestive tract,encompassing animals,plants,protists,and fungi,indicating that B.japonicum is omnivorous.At the phylum level,the dominant eukaryotes in the digestive tract of B.japonicum in autumn were Arthropoda(23.26%),Bacillariophyta(19.55%),and Chlorophyta(13.50%),whereas in spring,they were Chlorophyta(14.56%),Charophyta(13.96%),Arthropoda(8.45%),and Bacillariophyta(6.24%).Notably,the proportion of plant-based food was 38.10%in autumn and 35.86%in spring,while animal-based food constituted 32.98%in autumn and 14.17%in spring.At the order level,the relative abundance of Chlorellales increased in spring.Principal coordinate analysis(PCoA)and Non-Metric multidimensional scaling(NMDS)analyses indicated distinct differences in the composition and distribution of eukaryotic communities in the digestive tract of B.japonicum across seasons.This study provides a theoretical foundation for improving laboratory breeding and artificial restocking of B.japonicum.