寒喘祖帕对过敏性哮喘小鼠的抗炎效果及其对肠道菌群结构调节作用研究OA

Objective:This study aims to investigate the anti-inflammatory pharmacodynamic effects of Han-chuan Zupa on an allergic asthma(AAS)mouse model and its regulatory effects on the gut microbiota structure.Methods:An AAS mouse model was established using ovalbumin(OVA)sensitization and challenge.Mice were ad-ministered dexamethasone and hantazol via oral gavage.After three weeks of oral gavage,airway hyperresponsiveness(AHR)was assessed using an EMKA volume recorder.Blood samples and bronchoalveolar lavage fluid(BALF)were collected from the mice,and cell counts were analyzed.The levels of IgE,TNF-α,IL-4,IL-5,IL-6,and IL-13 in BALF and serum were detected using the ELISA method.Lung tissue was stained with hematoxylin and eosin(HE)to observe pathological changes in mouse lung tissue.The 16 S rRNA sequencing technique was used to analyze chan-ges in the intestinal microbiota of mice.Results:Hanchuan Zupa significantly improved the mental state,activity lev-el,and body weight of asthmatic mice(P<0.05),delayed the increase in Penh values,and reduced airway hyperre-sponsiveness(AHR).Pathological analysis indicated that Hanchuan Zupa alleviated tracheal epithelial damage,pul-monary interstitial thickening,and inflammatory infiltration.Gut microbiota analysis showed that Hanchuan Zupa significantly increased the Shannon and Simpson indices and restoredαdiversity of the microbiota.At the phylum level,Hanchuan Zupa increased the abundance of beneficial bacteria such as Firmicutes and Proteobacteria,while re-ducing the proportion of Bacteroidetes;at the genus level,nine bacterial communities,including Prevotellaceae_UCG_001 and Akkermansia,were identified as key biomarkers.Conclusion:Hanchuan Zupa alleviates asthma symptoms by regulating the structure and diversity of the gut microbiota.Its mechanism of action may be related to the regulation of the microbiota-immune axis,providing microbiological evidence for the use of Hanchuan Zupa in the treatment of asthm.