电针足三里对肝叶部分切除术后小鼠免疫功能及炎症反应的影响OA

Objective:To investigate the effects of electroacupuncture(EA)at Zusanli(ST36)on immune func-tion and inflammatory response in mice undergoing partial hepatectomy.Methods:C57 BL/6 mice were randomly allo-cated into a blank group,a model group,and an EA group using a computer-generated random number table.The model and EA groups underwent 70%partial hepatectomy under general anaesthesia to establish the surgical trauma model.The EA group received electroacupuncture at bilateral ST36 one day prior to surgery and on postoperative days 1,2 and 3.The blank and model groups did not receive EA treatment.On postoperative day 4,blood and spleen samples were collected from each group to measure cytokine levels(IL-4,IL-10)and lymphocyte subsets(CD4+T cells,CD8+T cells)to assess the impact of EA on postoperative immune function and inflammatory markers.The re-maining mice in each group were subjected to Pseudomonas aeruginosa nasal inoculation.Six hours later,half of the mice in each group were randomly selected for lung tissue collection,bacterial culture plating,and colony counting.The survival rates of the remaining mice were monitored for five days post-inoculation to evaluate their ability to clear secondary bacterial infections.Results:Compared with the blank group,the model group exhibited significantly elevated IL-4 and IL-10 levels,a marked reduction in splenic CD4+T cell proportion,a significant increase in lung bacterial colonies,and a notably lower post-infection survival rate on postoperative day 4(P<0.05).In contrast,the EA group demonstrated significantly lower serum anti-inflammatory cytokine(IL-4,IL-10)levels,an increased proportion of splenic CD4+T cells,fewer lung bacterial colonies,and improved post-infection survival rates compared to the model group(P<0.05).Conclusion:Electroacupuncture at ST36(Zusanli)alleviated immunosuppression in mice following partial hepatectomy and exerted a protective effect by reducing the postoperative inflammatory response.