基于miR-106b-5p/BTG3轴探讨胃衡汤对甲基硝基亚硝基胍诱导胃癌前病变小鼠的效应机制OA

Objective:To investigate the therapeutic effect of Weiheng decoction on MNNG-induced precancer-ous lesions of gastric cancer(PLGC)mice and its regulatory mechanism on miR-106b-5p/BTG3 axis.Methods:The PLGC mouse model was established by MNNG combined with irregular feeding.Sixty mice were randomly divided into blank group,model group,Weiheng decoction low,medium and high dose group and positive drug control group,with 10 mice in each group.The pathological changes of gastric mucosa were observed by HE staining.The expres-sion levels of TNF-α,IL-6,IL-1β,IL-10 and TGF-βwere detected by ELISA.The expression of BTG3,Ki67,Bax and Caspase protein was detected by Western blot.The ultrastructure was observed by transmission electron microscope.The expression of miR-106b-5p was detected by RT-qPCR,and its targeting relationship with BTG3 was verified.Results:Weiheng decoction can significantly improve the pathological manifestations of gastric mucosal atrophy,in-testinal metaplasia and inflammatory infiltration in PLGC mice.Compared with the model group,Weiheng decoction could significantly reduce the expression levels of pro-inflammatory factors TNF-α,IL-6 and IL-1β,and increase the level of anti-inflammatory factor IL-10.It significantly inhibited the expression of miR-106b-5p,up-regulated the ex-pression of its target gene BTG3 protein,decreased the expression of proliferation marker Ki67,and promoted the ex-pression of apoptotic proteins Bax,Caspase-3 and Caspase-9 in a dose-dependent manner,and the effect of high dose was the most significant.Conclusion:Weiheng decoction may relieve the inhibition of target gene BTG3 by down-reg-ulating miR-106b-5p/BTG3,thereby activating BTG3-mediated signaling pathway,inhibiting inflammatory response,promoting apoptosis and inhibiting abnormal proliferation,so as to improve PLGC.