沙地植被恢复类型对生物结皮微生物群落组成的影响OA

[Objective]This study aims to investigate the effects of different vegetation restoration types on the microbial community composition of biological soil crusts(biocrusts)in sandy ecosystems,providing a theoretical basis for the management of sandy ecosystems.[Methods]High-throughput sequencing technology was employed,with bare sandy land as the control,to analyze the variation characteristics of microbial community composition and diversity in biocrusts under different vegetation restoration types,including herbaceous,shrub,and symbiotic shrub and herb.[Results](1)Compared to bare sandy land where the dominant bacterial phylum was Proteobacteria and the dominant genus was Acinetobacter,the dominant bacterial phylum in biocrusts under the vegetation restoration types of herbaceous,shrub,and symbiotic shrub and herb was Cyanobacteria.The dominant bacterial genus was Microvirga under herbaceous and symbiotic shrub-herb types,while the dominant genus was RB41 under the vegetation restoration type of shrub.In contrast,the dominant fungal phyla in biocrusts across all vegetation restoration types were consistent with those in bare sandy land,namely Ascomycota and Basidiomycota.(2)All vegetation restoration types significantly increased the α-diversity of both bacterial and fungal communities in biocrusts.For bacterial communities,Shannon and Chao1 indices followed the order:symbiotic shrub and herb>shrub>herbaceous>bare sandy land.For fungal communities,the order was herbaceous>symbiotic shrub and herb>shrub>bare sandy land.(3)The β-diversity of bacterial communities was significantly influenced by vegetation restoration types,while the β-diversity of fungal communities appeared to be more affected by non-vegetation factors.[Conclusion]Vegetation restoration types significantly affect bacterial community composition and enhance microbial diversity in biocrusts,but exhibit no significant impact on fungal communities.