基于转录组测序探索雷公藤甲素与雷藤舒对类风湿关节炎滑膜成纤维细胞调控机制的异同OA

Objective To explore the molecular mechanisms by which triptolide(TP)and(5R)-5-hydroxytriptolide(T8)regulate fibroblast-like synoviocytes(FLS)in rheumatoid arthritis(RA)using transcriptome sequencing.Methods The synovial fibroblast cell line MH7A was intervened with TP and T8 respectively.After 24 h,the cells were collected and transcriptome sequencing was performed,and the sequencing results were analyzed by bioinformatics.Results Key genes are regulated by TP included tumor necrosis factor(TNF),toll-like receptor 2(TLR2),nuclear factor kappa B inhibitor alpha(NFKBIA),interleukin(IL)-6,and IL-1β.These genes are primarily involved in biological processes such as inflammatory response,cellular response to endotoxin,and endotoxin-mediated signal transduction.Relevant signaling pathways include NOD-like receptors(NLR)pathways.Key genes are regulated by T8 included TNF,NFKBIA,IL-6,IL-4,and IL-1β,associated with processes such as inflammatory response,positive regulation of fibrinolysis,and angiogenesis,as well as the IL-17 signaling pathway.Conclusions TP and T8 share some commonalities and differences in the specific targets and signaling pathways regulating RA-FLS.This provides additional data support for previous studies on TP and T8 in RA and offers new insights into the mechanism of T8 as a novel compound for RA treatment.