文冠果全基因组SNP鉴定及遗传结构分析OA

[Objective]To understand the genetic diversity of the main Xanthoceras sorbifolium cultivars(lines)planted in Shandong Province,clarify their kinship,and lay the foundation for the development of SNP markers for the identification and evaluation of Xanthoceras sorbifolium germplasm resources.[Methods]Based on the third-generation whole-genome rese-quencing technology,SNP markers of Xanthoceras sorbifolium were developed.Meanwhile,the genetic structure and genetic diversity of Xanthoceras sorbifolium germplasm resources were studied by using genetic similarity,phylogenetic analysis,pop-ulation clustering analysis and principal component analysis.[Results]A total of 137,029 high-quality SNP marker loci were screened from 15 Xanthoceras sorbifolium germplasm resources.Based on SNP markers,the genetic similarity coefficient be-tween each pair of varieties ranged from 0.3241 to 0.4538,with an average of 0.38.Both the phylogenetic tree and population clustering analysis divided the 15 Xanthoceras sorbifolium germplasm resources into two groups,while the population principal component analysis showed them as one group.The genetic structure analysis jointly indicated that the Xanthoceras sorbifoli-um germplasm resources in different regions of China were not strictly classified according to geographical distribution.[Con-clusion]The genetic diversity of Xanthoceras sorbifolium germplasm resources is relatively rich,and there is germplasm re-source exchange among different regions.The use of SNP markers can effectively identify Xanthoceras sorbifolium germplasm resources.