不同氮源浓度配比对新疆野苹果组培苗生长的影响及机制研究OA

[Objective]Tissue rapid propagation experiments of Malus sieversii(Xinjiang wild apple)were conducted to investigate the effects of different nitrogen source concentration ratios on its tissue culture growth,aiming to provide a scientific basis and theoretical reference for the conservation and utilization of Xinjiang wild apple germplasm resources as well as the es-tablishment of a high-efficiency in vitro propagation system.[Methods]Using tissue-cultured seedlings of Xinjiang wild apple as experimental material,five treatment groups were established:T1(100%NO-3),T2(75%NO-3+25%NH4+),T3(50%NO-3+50%NH4+),T4(25%NO-3+75%NH4+),and T5(100%NH4+).A systematic investigation was con-ducted to explore the effects of the concentration ratio of nitrate nitrogen(NO-3)to ammonium nitrogen(NH4+)in 1/2 MS medium on in vitro growth,root development,and physiological characteristics.[Results]The nitrogen source ratio signifi-cantly regulated the morphogenesis of the tissue-cultured seedlings.The T4 treatment demonstrated optimal performance in plant height(20.01 cm),root length(9.45 cm),root number(12),leaf number(22),and leaf thickness(0.11 mm).[Conclusion]These findings provide an optimized nitrogen nutrition strategy for the in vitro conservation and efficient rapid propagation of Malus sieversii germplasm resources.