漆树子代遗传多样性分析与指纹图谱构建OA

This study aims to investigate the genetic diversity of offspring of Toxicodendron vernicifluum families in Yunnan Province and promote the breeding and application of unique T.vernicifluum varieties.Thirty-six seed samples from 36 T.vernicifluum families were collected in Zhaotong City,Nujiang Prefecture,and Diqing Prefecture of Yunnan Province.The seeds were treated for germination and then sown,and tender leaves of the seedlings were collected for genomic DNA extraction.Simple sequence repeat-polymerase chain reaction(SSR-PCR)was used for amplification,followed by capillary electrophoresis and SSR for locus detection of the 36 T.vernicifluum offspring families.Genetic diversity was subsequently assessed,and SSRs were used to establish DNA fingerprints of the families.PCR revealed 26.667 observed alleles across 9 loci,with an average number of observed alleles being 2.963.The average number of effective alleles,average Shannon's information index,average observed heterozygosity,average expected heterozygosity,average polymorphic information content,and average gene flow were 2.280,0.835,0.527,0.484,0.534,and 2.131,respectively.Overall,the 36 T.vernicifluum offspring families showed high genetic similarity,and cluster analysis classified them into 3 groups.The offspring families of T.vernicifluum exhibited rich genetic diversity,with minimal genetic differentiation among populations and a relatively complex genetic background.Multi-locus matching analysis identified c26126,M66,M156,and Tox003 as core primers,which could be used to construct DNA fingerprints for the 36 T.vernicifluum offspring families.