醒神开窍合剂对颅脑损伤大鼠运动功能及自噬相关蛋白Beclin1、LC3-Ⅱ表达的影响OA

Objective:To investigate the effects of Xingshen Kaiqiao Compounds(XKC)on motor function and the expression of autophagy-related proteins Beclin1 and LC3-II in rats with traumatic brain injury(TBI).Methods:Thirty male Sprague-Dawley(SD)rats were randomly divided into three groups(n=10 per group):sham group,model group,and XKC group.The model and XKC groups were subjected to TBI using a pneumatic impact method,while the sham group underwent skin incision and skull exposure without impact.After modeling,the XKC group received intragastric administration of XKC(5.99 g/kg,divided into three doses daily),while the sham and model groups received equivalent volumes of saline.At 3 and 7 days after intervention,neurological function deficits were assessed using the modified Neurological Severity Score(mNSS),and motor function was evaluated via the rotarod test.After 7 days of intervention,serum lactate dehydrogenase(LDH)release rate was measured using a commercial kit.Hematoxylin-eosin(HE)staining was performed to assess neurohistological changes.Western blotting was used to detect the expression of Nestin,glial fibrillary acidic protein(GFAP),Beclin1,and LC3-II in injured brain tissues.Results:(1)mNSS evaluation results showed that on day 3 after intervention,both the model group and XKC group had significantly higher scores than the sham group(P<0.01),with no significant difference between the model and XKC groups(P>0.05).By day 7,the model and XKC groups remained significantly higher than the sham group(P<0.05,P<0.01),but the XNKQ group exhibited lower scores than the model group(P<0.05).(2)Rotarod test results demonstrated that on day 3,both the model and XKC groups had significantly lower scores than the sham group(P<0.01),with no difference between the two groups(P>0.05).By day 7,the model and XKC groups remained lower than the sham group(P<0.05,P<0.01),though the XNKQ group showed a trend toward higher scores than the model group,without statistical significance(P>0.05).(3)LDH release rate:Compared with the sham group,the model group showed significantly increased serum LDH release(P<0.05).The XKC group exhibited a marked reduction in LDH release compared to the model group(P<0.05),with no significant difference between the XKC and sham groups(P>0.05).(4)HE staining:The sham group displayed normal neuronal morphology with orderly cell arrangement.The TBI model group showed disordered cell arrangement,irregular morphology,and pyknotic nuclei.The XKC group demonstrated improved histological structure,with more regular cell arrangement and reduced nuclear pyknosis compared to the model group.(5)Western blot:Compared to the sham group,Beclin1 and LC3-II expression was significantly upregulated in the model group(P<0.05),while GFAP and Nestin expression showed a downward trend without statistical significance(P>0.05).Compared to the model group,the XKC group showed significantly increased GFAP and Nestin expression(P<0.05)and decreased Beclin1 and LC3-II expression(P<0.05).No significant differences were observed between the XKC and sham groups in Nestin,GFAP,Beclin1,or LC3-II expression(P>0.05).Conclusion:Xingshen Kaiqiao Compounds may improve limb function in TBI rats by enhancing the expression of Nestin and GFAP in injured brain tissues,reducing serum LDH release and autophagy-related proteins Beclin1 and LC3-II.