Mechanistic insights into Banxia Xiexin Decotion-induced gastric mucosal restoration in rats with chronic atrophic gastritis and gastric precancerous lesions:the role of the Notch pathwayOA
Background:The development of gastric cancer(GC)encompasses precancerous conditions like chronic atrophic gastritis(CAG)and premalignant lesions of gastric cancer(PLGC).In these situations,abnormal Notch signaling results in mucosal impairment and the initiation of cancer.Banxia Xiexin Decoction(BXD),a well-known formula in traditional Chinese medicine(TCM),shows promise in treating gastric disorders,but its mechanisms in gastric restoration remain unclear.Methods:Using MNNG-induced CAG and PLGC rat models,BXD was administered for 12 weeks.Gastric mucosal pathology was assessed via hematoxylin-eosin staining.Proliferation(Ki-67)and angiogenesis(VEGFA)markers were evaluated by immunohistochemistry.Network pharmacology identified BXD’s targets and pathways.Notch pathway components(Notch1,Jagged1,Dll4,Hes1)were analyzed via qPCR,Western blot,and immunohistochemistry.Results:BXD significantly ameliorated mucosal atrophy,glandular structural disorder,and dysplasia in CAG and PLGC rats.Network pharmacology revealed 323 overlapping targets between BXD and PLGC,with Notch signaling as a central pathway.BXD downregulated Notch1,Jagged1,Dll4,and Hes1 expression at transcriptional and protein levels,suppressed Ki-67(proliferation)and VEGFA(angiogenesis)overexpression,and restored gastric mucosal integrity.Conclusion:BXD inhibits Notch signaling,reduces aberrant proliferation and angiogenesis,and interrupts Correa’s gastric carcinogenesis cascade.This study provides mechanistic evidence supporting BXD as a TCM-based intervention for gastric precancerous lesions.
Jia-Le Ma;Xin-Yuan Zhang;Yu-Yao Wang;Meng-Tong Leng;Hui-Zhen Li;Tai-Yi Wang
Department of Gastroenterology,The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 301600,ChinaDepartment of Gastroenterology,The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 301600,ChinaInstitute of Acupuncture and Moxibustion,Shandong University of Traditional Chinese Medicine,Jinan 250355,ChinaDepartment of Gastroenterology,The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 301600,ChinaDepartment of Gastroenterology,The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 301600,ChinaInstitute of Acupuncture and Moxibustion,Shandong University of Traditional Chinese Medicine,Jinan 250355,China Shandong Key Laboratory of Innovation and Application Research in Basic Theory of Traditional Chinese Medicine,Shandong University of Traditional Chinese Medicine,Jinan 250355,China Key Laboratory of Traditional Chinese Medicine Classical Theory,Ministry of Education,Shandong University of Traditional Chinese Medicine,Jinan 250355,China
医药卫生
Banxia Xiexin Decotionchronic atrophic gastritisgastric precancerous lesionspremalignant lesions of gastric cancerNotch signaling pathway
《Traditional Medicine Research》 2026 (4)
P.27-38,12
supported by the National Natural Science Foundation of China(Grant No.82274442)the Key Research Project in Traditional Chinese Medicine of Tianjin Municipal Health Commission(Grant No.202007)the Integrated Traditional Chinese and Western Medicine Research Project of Tianjin Municipal Health Commission(Grant No.2023134).
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