Epigenetic regulation of JASMONATE ZIM-DOMAIN genes contributes to heat tolerance in the heat-tolerant rice cultivar Nagina 22OA
Extreme hot weather severely limits rice(Oryza sativa)production.Rice cultivars from regions with hot weather are a valuable resource for breeding heat-tolerant rice,but the mechanisms mediating heat tolerance in these cultivars are not fully understood.Here,we investigated heat-tolerance mechanisms in rice using the well-known heat-tolerant cultivar Nagina 22(N22)and comparing it with the less heat-tolerant cultivar 93–11.Following heat shock(HS)treatment(45℃ for 3 or 24 h),the expression of JASMONATE ZIM-DOMAIN(JAZ)genes spiked during the early stages of HS responses in N22 but not 93–11 and genes related to jasmonic acid(JA)signaling were repressed in N22.Promoting JA signaling in N22 via pretreatment with methyl JA(MeJA)impaired the heat tolerance of N22,measured as survival after HS treatment of 45℃ for 30 h,followed by a 7-d recovery.Furthermore,the N22-specific activation of JAZ genes was associated with increased histone acetylation and decreased DNA methylation.Comparing N22 to 93–11,we propose that the DNA demethylation process,rather than the hypomethylation status per se,is likely associated with JAZ activation.In summary,we revealed epigenetic mechanisms that may contribute to the heat tolerance of N22 via the JA signaling pathway;our findings have implications for improving heat tolerance in rice and other crops.
Xiaoxuan Du;Yingnan Sun;Yonggang He;Haiya Cai;Xiangsong Chen
State Key Laboratory of Hybrid Rice,College of Life Sciences,Wuhan University,Wuhan 430072,China Hubei Hongshan Laboratory,Wuhan 430070,ChinaState Key Laboratory of Hybrid Rice,College of Life Sciences,Wuhan University,Wuhan 430072,China Hubei Hongshan Laboratory,Wuhan 430070,ChinaHubei Key Laboratory of Food Crop Germplasm and Genetic Improvement,Key Laboratory of Crop Molecular Breeding,Ministry of Agriculture and Rural Affairs,Food Crops Institute,Hubei Academy of Agricultural Sciences,Wuhan 430064,China Scientific Observation and Experiment Station for Crop Gene Resources and Germplasm Enhancement in Hubei,Ministry of Agriculture and Rural Affairs,Wuhan 430064,ChinaHubei Key Laboratory of Food Crop Germplasm and Genetic Improvement,Key Laboratory of Crop Molecular Breeding,Ministry of Agriculture and Rural Affairs,Food Crops Institute,Hubei Academy of Agricultural Sciences,Wuhan 430064,China Scientific Observation and Experiment Station for Crop Gene Resources and Germplasm Enhancement in Hubei,Ministry of Agriculture and Rural Affairs,Wuhan 430064,ChinaState Key Laboratory of Hybrid Rice,College of Life Sciences,Wuhan University,Wuhan 430072,China Hubei Hongshan Laboratory,Wuhan 430070,China
农业科技
HeatNagina 22JAZDNA methylation
《aBIOTECH》 2025 (3)
P.441-451,11
supported by Hubei Provincial Natural Science Foundation(2024AFA062,2022CFA025)National Key Technologies R&D Program(2023YFF1002400).
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