Regulatory roles of RNA modifications in plant development and fruit ripeningOA
The emerging field of epitranscriptomics has revolutionized our understanding of post-transcriptional regulation in plant systems.This review focuses on cutting-edge discoveries in the area of RNA modification,with a particular emphasis on the N^(6)-methyladenosine(m^(6)A)-mediated regulatory networks that govern plant development and fruit maturation.We systematically summarize the spatiotemporal patterns of RNA modifications and their integration into phytohormone signaling cascades and responses to environmental stimuli.Advanced epitranscriptome sequencing platforms have identified evolutionarily conserved modification signatures across angiosperm species,while simultaneously revealing species-specific regulatory architectures.Despite substantial progress,our understanding of the molecular mechanisms that underlie RNA modifications,especially those other than m^(6)A,remains limited.We propose an innovative roadmap that combines CRISPR-based writer/eraser manipulation,single-cell spatial epitranscriptomics,and synthetic biology approaches to harness RNA modification networks for precision agriculture.We also underscore the importance of interdisciplinary collaboration that integrates findings from biology,chemistry,physics,and computer science to decode the plant epitranscriptome.To enable precise control of postharvest physiology,future priorities should include the development of biosensors for specific modification types,the engineering of RNA modification–dependent translation control systems,and the development of RNA epigenetic editing tools.
Tianxiang Li;Junmei Huang;Guanqun Wang;Haoxuan Li;Peitao Lü
National Key Laboratory of Tropical Crop Breeding,Institute of Tropical Bioscience and Biotechnology&Sanya Research Institute,Chinese Academy of Tropical Agricultural Sciences,Sanya 572024,China College of Horticulture,Center for Plant Metabolomics,Haixia Institute of Science and Technology,Fujian Agriculture and Forestry University,Fuzhou 350002,ChinaNational Key Laboratory of Tropical Crop Breeding,Institute of Tropical Bioscience and Biotechnology&Sanya Research Institute,Chinese Academy of Tropical Agricultural Sciences,Sanya 572024,China College of Horticulture,Center for Plant Metabolomics,Haixia Institute of Science and Technology,Fujian Agriculture and Forestry University,Fuzhou 350002,ChinaSchool of Life Sciences and State Key Laboratory of Agrobiotechnology,The Chinese University of Hong Kong,Shatin,Hong KongDepartment of Chemistry,Department of Biochemistry and Molecular Biology,and Institute for Biophysical Dynamics,The University of Chicago,Chicago,IL 60637,USANational Key Laboratory of Tropical Crop Breeding,Institute of Tropical Bioscience and Biotechnology&Sanya Research Institute,Chinese Academy of Tropical Agricultural Sciences,Sanya 572024,China
农业科技
EpigeneticsEpitranscriptomicsFruit maturationPlant development
《aBIOTECH》 2025 (3)
P.472-488,17
supported by the National Natural Science Foundation of China(32372666)the Project of the National Key Laboratory of Tropical Crop Breeding(NKLTCB-RC202402 and NKLTCBCXTD26)the Central Public-interest Scientific Institution Basal Research Fund for the Chinese Academy of Tropical Agricultural Sciences(1630052024024).
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