Cis-regulatory elements:systematic identification and horticultural applicationsOA
Cis-regulatory elements(CREs)are the genetic DNA fragments bound by transcription factors(TFs).CREs function as molecular switches that precisely modulate the dosage and spatiotemporal patterns of gene expression.The systematic identification of CREs not only facilitates the annotation of the functional non-coding genome but also provides essential insights into the architecture of gene regulatory networks and sheds light on an accurate selection of the target sites for genetic engineering of crops.In this review,we summarize the current high-throughput methodologies used for identifying CREs,illustrate the associations between CREs and agronomic traits in horticultural crops,and discuss how CREs can be exploited to facilitate crop breeding.
Tian Li;Wen Zeng;Fangjie Zhu;Peitao Lü
Haixia Institute of Science and Technology,Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology,College of Horticulture,National Engineering Research Center of JUNCAO,Fujian Agriculture and Forestry University,Fuzhou 350002,ChinaHaixia Institute of Science and Technology,Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology,College of Horticulture,National Engineering Research Center of JUNCAO,Fujian Agriculture and Forestry University,Fuzhou 350002,China National Key Laboratory of Tropical Crop Breeding,Institute of Tropical Bioscience and Biotechnology&Sanya Research Institute,Chinese Academy of Tropical Agricultural Sciences,Sanya 572024,ChinaHaixia Institute of Science and Technology,Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology,College of Horticulture,National Engineering Research Center of JUNCAO,Fujian Agriculture and Forestry University,Fuzhou 350002,ChinaNational Key Laboratory of Tropical Crop Breeding,Institute of Tropical Bioscience and Biotechnology&Sanya Research Institute,Chinese Academy of Tropical Agricultural Sciences,Sanya 572024,China
农业科技
BreedingCis-regulatory elementDNA-binding specificityEpigeneticsGene regulatory networkHorticultural crops
《aBIOTECH》 2025 (3)
P.510-527,18
supported by the National Natural Science Foundation of China(32170554,32372666,32370582)the National Key Research and Development Program of China(2024YFC3407200)the Project of National Key Laboratory of Tropical Crop Breeding(NKLTCB-RC202402 and NKLTCBCXTD26)“Chu Ying”Young Talent Project of Fujian,Funding for High-level Overseas Personnel(Ministry of Human Resources and Social Security).
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