苎麻BnGCL1基因响应干旱胁迫的功能研究OA
Functional study of the BnGCL1 gene in ramie(Boehmeria nivea L.)in response to drought stress
干旱是影响植物生长发育的主要逆境之一.本研究探讨了苎麻(Boehmeria nivea L.)BnGCL1 基因在干旱胁迫响应中的功能机制.结果表明,BnGCL1 基因序列最大开放阅读框(ORF)为 1581 bp,编码 526 个氨基酸;蛋白的等电点为 5.79,分子量为 59,123.98 Da,脂肪系数为 78.78,稳定系数为 37.42,属于稳定蛋白.BnGCL1 基因在苎麻的根、茎、叶中均有表达,受干旱胁迫诱导表达.在干旱条件下,过表达BnGCL1 植株的根长、鲜重、叶绿素a、叶绿素b均显著高于野生型,同时抗氧化酶如APX和γ-GCL的活性以及渗透调节相关物质如GSSG、Pro等的代谢水平均发生显著变化.BnGCL1 的过表达显著上调AtGST1、AtGST11、AtNCED3 和AtWRKY40 等干旱应答相关基因的表达水平,表明 BnGCL1 通过调控抗氧化系统和干旱响应信号通路参与植物的抗旱应答.通过 VIGS 技术验证了BnGCL1 基因的沉默能够降低苎麻的耐旱性,表明其在苎麻干旱响应中的作用.本研究揭示了 BnGCL1 基因在干旱胁迫中的重要作用,为苎麻的抗旱分子机制研究和抗旱品种培育提供了理论依据.
Drought is one of the major environmental stresses that affects plant growth and development.In this study,the func-tional role of the BnGCL1 gene in the drought stress response of ramie(Boehmeria nivea L.)was investigated.The results showed that the gene contains a maximum open reading frame(ORF)of 1581 bp,encoding a protein of 526 amino acids.The predicted protein has an isoelectric point of 5.79,a molecular weight of 59,123.98 Da,a fat index of 78.78,and an instability index of 37.42,indicating that it is a stable protein.BnGCL1 is expressed in the roots,stems,and leaves of ramie,and its expression is induced by drought stress.Under drought conditions,transgenic plants overexpressing BnGCL1 exhibited significantly greater root length,fresh weight,chlorophyll a,and chlorophyll b contents compared to wild-type plants.In addition,the activities of antioxidant enzymes such as APX and γ-GCL,as well as the levels of osmotic regulators including GSSG and Pro,were significantly altered.Overexpression of BnGCL1 also markedly upregulated the expression of drought-responsive genes,including AtGST1,AtGST11,AtNCED3,and AtWRKY40,suggesting that BnGCL1 enhances drought tolerance by modulating the antioxidant defense system and drought-responsive signaling pathways.Gene silencing experiments using VIGS technology further confirmed that suppres-sion of BnGCL1 reduces drought tolerance in ramie,highlighting its critical role in drought response.This study reveals the im-portant function of BnGCL1 under drought stress and provides a theoretical foundation for elucidating the molecular mechanisms of drought tolerance and for breeding drought-resistant ramie varieties.
刘海波;张蕾;王立琦;石晓丽;周文莹;崔国贤;佘玮
湖南农业大学苎麻研究所,湖南 长沙 410128||湖南农业大学农学院,湖南 长沙 410128湖南农业大学苎麻研究所,湖南 长沙 410128||湖南农业大学农学院,湖南 长沙 410128湖南农业大学苎麻研究所,湖南 长沙 410128||湖南农业大学农学院,湖南 长沙 410128湖南农业大学苎麻研究所,湖南 长沙 410128||湖南农业大学农学院,湖南 长沙 410128湖南农业大学苎麻研究所,湖南 长沙 410128||湖南农业大学农学院,湖南 长沙 410128湖南农业大学苎麻研究所,湖南 长沙 410128||湖南农业大学农学院,湖南 长沙 410128湖南农业大学苎麻研究所,湖南 长沙 410128||湖南农业大学农学院,湖南 长沙 410128
苎麻BnGCL1干旱胁迫抗氧化酶分子机制
Boehmeria nivea L.BnGCL1drought stressantioxidant enzymesmolecular mechanism
《作物学报》 2026 (1)
14-27,14
本研究由财政部和农业农村部国家现代农业产业技术体系建设专项(麻类,CARS-16-E11)资助. This study was supported by the China Agriculture Research System of MOF and MARA(Fibre,CARS-16-E11).
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