多重荧光PCR法鉴别半夏药材及饮片中的掺混伪品OA北大核心

Objective:To establish a multiplex fluorescent PCR method for identification of Pinellia ternate and its common counter-feits Pinellia pedatisecta,Typhonium flagelliforme,Arisaema erubescens,and to verify its feasibility,so as to provide methodological support for formulating the quality standard of Pinellia ternate.Methods:The multiplex PCR system was optimized,and the specificity,sensitivity,detection limit of the method were investigated.The 15 batches of medicinal materials and decoction pieces suspeeted to be Pinellia ternate counterfeit in the earlier stage were tested and verified.Results:The method established in this study detected 8 batches of Pinellia pedati-secta,3 batches of Typhonium flagelliforme and 4 batches of non-Pinellia ternate other confounding products,and the measured results were completely consistent with the sequencing results.The method had no cross-reaction in the common related species of the same family,and only needed the template amount of pg DNA to detect,and could detect the mixed counterfeits with only 5%adulteration rate.Con-clusion:The established multiplex fluorescent PCR method has the advantages of sensitivity,speed and accuracy,and can be widely used as a specific detection method for Pinellia ternate medicinal materials and decoction pieces and its common counterfeits.