基于HPLC指纹图谱和多成分定量评价桑寄生质量OA北大核心

Objective:To establish the HPLC fingerprint of Taxilli Herba and the determination methods of gallic acid,avicularin,quercitrin,catechin,isoquercitrin and epicatechin,to evaluate the quality of Taxilli Herba from different habitats.Methods:The chroma-tography was performed on the GL Sciences InertSustain AQ-C18(250 mm×4.6 mm,5 μm)chromatographic column,the mobile phase was acetonitrile-0.1%phosphoric acid aqueous solution with gradient elution,detection wavelength was 230 nm,column temperature was 30℃,flow rate was 1.0 mL/min,and injection volume was 10 μL.The HPLC fingerprint of Taxilli Herba was constructed,and the con-tents of six components were simultaneously determined.A combinations of cluster analysis(CA),principal component analysis(PCA),orthogonal partial least squares discriminant analysis(OPLS-DA)and entropy weight-TOPSIS analysis was used to evaluate the quality.Results:HPLC fingerprint of Taxilli Herba was established and 14 common peaks were calibrated.5 markers of quality difference were confirmed.The results of entropy weight-TOPSIS analysis showed that the quality of Taxilli Herba from Guangdong,Guangxi was better.The contents of gallic acid,catechin,epicatechin,isoquercetin,avicularin and quercetin were significantly different in different areas.Conclusion:The HPLC fingerprint and content determination method of Taxilli Herba is reasonable and reliable.Chemometrics combined with entropy weight-TOPSIS method can be used to evaluate the quality of Taxilli Herba and provide basis for its quality control.