野八角果实的化学成分研究OA北大核心

Objective:To study the chemical constituents from the fruits of Illicium simonsii.Methods:The compounds from the eth-yl acetate fraction of the ethanol extract of Illicium simonsii fruits were isolated and purified by silica gel column chromatography,Sepha-dex LH-20 gel column chromatography,RP-C18 column chromatography and semi-preparative high performance liquid chromatography,and the structures of the compounds were identified by spectral data and literature.The in vitro anti-human colon cancer cell(HCT-116)activity of the compounds was tested by MTT assay.Results:Eighteen compounds were isolated from the fruits of Illicium simonsii and i-dentified as henrylactone A(1),oligandrumin D(2),veranisatin D(3),blumenol A(4),blumenol B(5),prinsepiol(6),7S,8R-di-hydrodehydrodiconiferyl alcohol(7),sinensiol G(8),dehydrodiconiferyl alcohol(9),rhoifoline B(10),4-methoxy-N-methyl-2-quinolo-ne(11),trans-N-p-coumaroyltyramine(12),p-hydroxybenzaldehyde(13),vanillyl alcohol(14),quercetin(15),mexoticin(16),samwi-rin(17),(-)-massoniresinol(18),respectively.In vitro experiments showed that compounds 1 and 11 had inhibitory effect on HCT-116 cells.Conclusion:Among them,compounds 4 to 6,8,10 to 12,14,16,17 are isolated from Illicium genus for the first time,compound 18 is isolated from Illicium simonsii for the first time.Compounds 1 and 11 show potential antitumor activity with IC50 of(3.18±0.11)μmol/L and(2.43±0.12)μmol/L,respectively.