331份花生种质苗期耐盐性综合评价和强耐盐种质鉴选OA
Comprehensive evaluation of salt tolerance and identification of elite salt-tolerant germplasm in 331 peanut accessions at seedling stage
土壤盐渍化是限制花生扩种及产量的主要制约因素之一,筛选优异耐盐花生种质并鉴定相关指标,为耐盐品种选育和耐盐分子机制研究奠定基础.以 331 份花生种质为研究对象,利用水培法于苗期进行耐盐性鉴定.测定了 8 个指标:SPAD值、苗高、地上鲜重、地下鲜重、地上干重、地下干重、鲜重根冠比和干重根冠比.采用主成分分析法、隶属函数分析法及聚类分析法对花生苗期耐盐性进行综合评价.在不同盐浓度处理下,苗高、地上鲜重、地下鲜重、地上干重、鲜重根冠比和干重根冠比 6 个指标在不同种质间均表现出显著差异.在盐胁迫下,花生生长受抑制,其中 4 个指标的测定值较对照显著降低.相关性分析显示,8 个指标的耐盐系数均存在相关性,其中地下鲜重和地下干重的相关性最强(相关系数为0.83).主成分分析将8个指标转换成3个主成分,累计方差贡献率达76.22%.基于D值进行聚类分析,将 331 份种质划分为 5 类耐盐型,第Ι类强耐盐型(11 份)、第II类耐盐型(33 份)、第III类中间型(104 份)、第IV类盐敏感型(42 份)、第V类高盐敏感型(141 份).通过逐步回归分析,构建花生苗期耐盐评价回归方程:Y=0.032+0.163X4+0.137X3+0.073X1-0.158X2+0.111X5+0.080X6.D值适用于花生苗期耐盐性评价,筛选出包括华实 2、AM-Georganic、中花 6 等在内的 11 份强耐盐性种质.苗高、地下干重和地上干重可作为花生苗期耐盐性鉴定的主要指标.
Soil salinization is a major constraint limiting the expansion and yield of peanut cultivation.Screening salt-tolerant peanut germplasm and identifying related traits provide a foundation for breeding salt-tolerant varieties and investigating the mechanisms underlying salt tolerance.In this study,the salt tolerance of 331 peanut accessions was evaluated at the seedling stage using a hydroponic system.Eight parameters were measured:SPAD value,plant height,shoot fresh weight,root fresh weight,shoot dry weight,root dry weight,fresh weight root/shoot ratio,and dry weight root/shoot ratio.A comprehensive evaluation of salt tolerance was conducted using principal component analysis(PCA),membership function analysis,and cluster analysis.Un-der different salt concentrations,six parameters—plant height,shoot fresh weight,root fresh weight,shoot dry weight,fresh weight root/shoot ratio,and dry weight root/shoot ratio—showed significant differences among accessions.Salt stress inhibited peanut growth and significantly reduced four of these parameters compared with the control.Correlation analysis revealed significant relationships among the salt tolerance coefficients of all eight parameters,with the strongest correlation observed be-tween root fresh weight and root dry weight(r=0.83).PCA reduced the eight parameters to three principal components,account-ing for a cumulative variance of 76.22%.Based on D-values(comprehensive evaluation scores),cluster analysis grouped the 331 accessions into five categories:Group I(highly salt-tolerant,11 accessions),Group II(salt-tolerant,33 accessions),Group III(intermediate,104 accessions),Group IV(salt-sensitive,42 accessions),and Group V(highly salt-sensitive,141 accessions).A stepwise regression analysis yielded a predictive equation for evaluating salt tolerance in peanut seedlings:Y=0.032+0.163X4+0.137X3+0.073X1-0.158 X2+0.111X5+0.080X6.The D-value proved effective for assessing salt tolerance at the seedling stage,and 11 salt-tolerant germplasms,including Huashi 2,AM-Ceorganic,and Zhonghua 6,were identified.Plant height,root dry weight,and shoot dry weight were identified as key indicators for evaluating peanut salt tolerance at the seedling stage.
王菲菲;陈静;张胜忠;杨贵华;苗华荣;胡晓辉;张则林;刘莎莎;乔利仙;单世华
山东省花生研究所,山东 青岛 266100山东省花生研究所,山东 青岛 266100山东省花生研究所,山东 青岛 266100济南市农业技术推广服务中心,山东 济南 250002山东省花生研究所,山东 青岛 266100山东省花生研究所,山东 青岛 266100滦州市油榨镇人民政府,河北 唐山 063702青岛鲁聚丰种业有限公司,山东 青岛 266200青岛农业大学农学院,山东 青岛 266109山东省花生研究所,山东 青岛 266100
花生苗期耐盐评价主成分分析隶属函数分析种质鉴选
peanutseedling stageassessment of salt toleranceprincipal component analysissubordinate function analysisgermplasm evaluation
《作物学报》 2026 (1)
279-294,16
本研究由山东省农业良种工程项目(2024LZGC031)和山东省农业科学院创新工程项目(CXGC2025E02)资助. This study was supported by the Shandong Province Agriculture Improved Seed Project(2024LZGC031)and the Innovation Project of Shandong Academy of Agriculture Sciences(CXGC2025E02).
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