径向超声小探头技术联合SHOX2、RASSF1A基因甲基化检测在肺外周实性/亚实性结节中的诊断作用研究OA

Objective:To investigate the diagnostic value of combining radial ultrasound small probe technology with Ras-related region family 1A(RASSF1A)gene and short homeobox gene 2(SHOX2)methylation detection in the diagnosis of peripheral solid/subsolid nodules in the lung.Methods:A total of 302 patients with peripheral pulmonary nodules admitted to our hospital from July 2021 to October 2023 underwent lung biopsy guided by the radial ultrasound small probe technique via bronchoscopy,and the methylation status of RASSF1A and SHOX2 genes in bronchoalveolar lavage fluid was detected by real-time fluorescence PCR.Results:Compared with radial ultrasound probe,RASSF1A,SHOX2 gene methylation alone,the combined diagnostic sensitivity of 99.45%,specificity of 100.00%,accuracy of 99.67%were higher(P<0.05).Compared with cytology,the sensitivity and specificity of the diagnosis of malignant pulmonary nodules increased from 50.82%to 99.45%and from 97.48%to 100.00%with the addition of RASSF1A and SHOX2 gene methylation.The positive rate of methylation of RASSF1A and SHOX2 genes was 100.00%in the cytological detection group,100.00%in the unknown group and 43.10%in the undetected group,which could effectively improve the detection rate.The detection rate of RASSF1A and SHOX2 gene methylation on subsolid malignant pulmonary nodules was higher than that of radial ultrasound probe(P<0.05).Conclusion:The radial ultrasound small probe technology combined with RASSF1A and SHOX2 gene methylation detection can effectively improve the detection rate of peripheral solid/subsolid nodules in the lung,and provide a reference for early clinical screening.