Ultrasound/magnetic field dual-responsive Fe_(3)O_(4)/glucose oxidase catalytic microbubbles for enhanced bacterial biofilm eliminationOA
Background:The bacterial biofilm poses a significant challenge to traditional antibiotic therapy.There is a great need to develop novel antibiofilm agents combined with biofilm disrupting and bacteria-killing without the dependence of antibiotic.Methods:Herein,we prepared ultrasound/magnetic field-responsive ferroferric oxide nanoparticles(Fe_(3)O_(4))/glucose oxidase microbubbles(FGMB)to form a cascade catalytic system for effective removing methicillin-resistant Staphylococcus aureus biofilms.FGMB were prepared through interfacial self-assembly of Fe_(3)O_(4) nanoparticles(NPs)and glucose oxidase(GOx)at the gas-liquid interface stabilized by surfactants.Under ultrasound/magnetic field stimulation,FGMB disrupted biofilm architecture through microbubble collapse-induced microjets and magnetically driven displacement.Simultaneously,ultrasound-triggered rupture of FGMB released GOx and Fe_(3)O_(4) NPs.Glucose can be oxidized by GOx to generate gluconic acid and hydrogen peroxide which was subsequently catalyzed into hydroxyl radicals by Fe_(3)O_(4) NPs,enabling chemical eradication of biofilm-embedded bacteria.Results:Optical microscopy images demonstrated that FGMB have spherical structure with average size of approximately 17μm.FGMB showed a 65.4%decrease in methicillin-resistant Staphylococcus aureus biofilm biomass and 1.1 log bacterial inactivation efficiency(91.2%),suggesting effective biofilm elimination.In vitro experimental results also indicate that FGMB have good biocompatibility.Conclusion:This antibiofilm strategy integrated dual modes of physical biofilm disruption with chemical bacteria-killing shows great potential as a versatile,non-resistant strategy for bacterial biofilm elimination.
Chi Zhang;Dou Wang;Liang Lu;Feng-Jiao Xu;Fan-Sen Xu;Xuan Wu;Xiao-Xiao Xu;Xiao Li;Li-Hui Yuwen
Jiangsu Key Laboratory of Smart Biomaterials and Theranostic Technology,Institute of Advanced Materials(IAM),Nanjing University of Posts and Telecommunications,Nanjing 210023,ChinaJiangsu Key Laboratory of Smart Biomaterials and Theranostic Technology,Institute of Advanced Materials(IAM),Nanjing University of Posts and Telecommunications,Nanjing 210023,ChinaJiangsu Key Laboratory of Smart Biomaterials and Theranostic Technology,Institute of Advanced Materials(IAM),Nanjing University of Posts and Telecommunications,Nanjing 210023,ChinaJiangsu Key Laboratory of Smart Biomaterials and Theranostic Technology,Institute of Advanced Materials(IAM),Nanjing University of Posts and Telecommunications,Nanjing 210023,ChinaJiangsu Key Laboratory of Smart Biomaterials and Theranostic Technology,Institute of Advanced Materials(IAM),Nanjing University of Posts and Telecommunications,Nanjing 210023,ChinaJiangsu Key Laboratory of Smart Biomaterials and Theranostic Technology,Institute of Advanced Materials(IAM),Nanjing University of Posts and Telecommunications,Nanjing 210023,ChinaJiangsu Key Laboratory of Smart Biomaterials and Theranostic Technology,Institute of Advanced Materials(IAM),Nanjing University of Posts and Telecommunications,Nanjing 210023,ChinaSchool of Electronic Information,Nanjing Vocational College of Information Technology,Nanjing 210023,ChinaJiangsu Key Laboratory of Smart Biomaterials and Theranostic Technology,Institute of Advanced Materials(IAM),Nanjing University of Posts and Telecommunications,Nanjing 210023,China
医药卫生
bacterial biofilmultrasoundmagnetic fieldcatalytic microbubblesmechanical disruptionchemical degradation
《Biomedical Engineering Communications》 2026 (1)
P.4-12,9
supported by the National Natural Science Foundation of China(22375101)the Natural Science of Colleges and Universities in Jiangsu Province(24KJB430027).
评论