藻类色素提取方式的比较研究OA北大核心

Algal pigments participate in photosynthesis by absorbing and transferring light energy.The composition and content of pigments vary among algae.Therefore,pigment analysis is crucial for studying photosynthetic physiology and identifying algal taxa.It has been widely applied in ecological explorations of the phytoplankton community composition and biomass assessment.Many extraction and analytical methods for algal pigments have been developed.This study compared five cell disruption and pigment extraction methods—sonication,manual grinding,freeze grinding,manual grinding combined with sonication,and freeze grinding combined with soni-cation—in micro-and macro-algae.The major findings were:(1)Sonication was particularly effective in extracting pigments from diatoms with rigid silica cell walls,unlike grinding and sonication in dinoflagellates without cell walls.(2)The five methods had notable variations in pigment extraction efficiency from two species of macroalgae,especially the brown alga Sargassum horneri.Manual grinding was more effective than freeze-grinding in the green alga Ulva prolifera,unlike in S.horneri.Sonication could further improve elicitation from S.horneri.(3)Among the five methods,freeze grinding combined with sonication was most effective in pigment extraction from micro-and macro-algal samples.Such a protocol is easy to operate,efficient,and time-saving.It presents wide applicabil-ity in future studies.