药用蜘蛛棒毛络新妇及其混淆品的分子鉴定OA北大核心

Objective:To establish a molecular identification method that can accurately identify Trichonephila clavata and its con-fused products to solve the confusion of medicinal materials and the hidden danger of drug use caused by the diversity of spider species.Methods:DNA was extracted from 70 samples,COⅠ sequence characteristics were analyzed,intraspecious and interspecific genetic dis-tances were calculated,and ML phylogenetic trees were constructed to evaluate the validity of COⅠ sequence as an identification meth-od of Trichonephila clavata and its confounding products.R language mapping software was used to convert molecular barcodes into QR codes,establish a dedicated DNA barcode library,and verify 8 commercially available spiders.Results:The length of COⅠ sequence of Trichonephila clavata was 603 bp,the content of GC was 31.8%to 32.5%,there were 16 base variation sites,and there were 14 haplo-types(Hd=0.8236),indicating that the sample population had rich diversity.The average interspecific genetic distance(0.1685)was significantly greater than the average intrasecific genetic distance(0.0034),which was consistent with the DNA Barcoding Gap test re-sults,indicating that there was a certain DNA barcode gap between COⅠ sequence and other species.In the constructed phylogenetic tree,different species were grouped into single branches.Among the 8 commercial samples verified by actual measurement,6 samples were identified as Trichonephila clavata,and 2 counterfeits were also accurately identified,with an accuracy of 100%.Conclusion:The molecular identification method of COⅠ sequence established in this study is highly specific,and can effectively solve the species iden-tification problem of the complete herbal medicine and its powder of Trichonephila clavata.