不同年限茅苍术中主要代谢物分布差异的比较OA北大核心

Objective:To compare the distributional difference of characteristics,main primary metabolites,volatile oil and main active components of Atractylodes lancea in different years,so as to provide scientific basis for elucidating the distribution characteristics and accumulation rules of active components of Atractylodes lancea.Methods:The section,texture and oil chamber density of herbs in different years were andyzed by visual and quantitative morphological methods.The soluble protein,soluble sugar and starch,total vola-tile oil contents were determined by Coomassie bright blue staining,anthraquinone colorimetric,steam distillation methods respectively.The contents of characteristic components such as atractylodin and atractylon were determined by HPLC and GC-MS.Results:With the increase of years,the color of the section was deepened,the texture was harder,the oil chamber in the section was enlarged,color was deepened,the ratio of oil chamber area to total area increased year by year.The density of fresh medicinal materials decreased with the increase of years,and the density of dry medicinal materials increased with the increase of years.The contents of soluble protein and sol-uble sugar decreased with the increase of years,and the contents of starch gradually increased with the increase of years.With the in-crease of years,the total volatile oil and atractylodin contents in the medicinal materials gradually increased,while the contents of atrac-tylon and β-eucalyptol gradually decreased.However,there was no significant difference in the content of hinesol.Conclusion:There are significant differences in the spatial distribution and content of primary and secondary metabolites in different age groups.The results provide theoretical support for further study on the mechanism of the influence of growth age on the quality of Atractylodes lancea.