Applying conventional and cell-type-specific CRISPR/Cas9 genome editing in legume plantsOA
The advent of genome editing technologies,particularly CRISPR/Cas9,has significantly advanced the generation of legume mutants for reverse genetic studies and understanding the mechanics of the rhizobial symbiosis.The legume–rhizobia symbiosis is crucial for sustainable agriculture,enhancing nitrogen fixation and improving soil fertility.Numerous genes with a symbiosis-specific expression have been identified,sometimes exclusively expressed in cells forming infection threads or in nitrogen-fixing nodule cells.Typically,mutations in these genes do not affect plant growth.However,in some instances,germline homozygous mutations can be lethal or result in complex pleiotropic phenotypes that are challenging to interpret.To address this issue,a rhizobia-inducible and cell-type-specific CRISPR/Cas9 strategy was developed to knock-out genes in specific legume transgenic root tissues.In this review,we discuss recent advancements in legume genome editing,highlighting the cell-type-specific CRISPR system and its crucial applications in symbiotic nitrogen fixation and beyond.
Jin-Peng Gao;Yangyang Su;Suyu Jiang;Wenjie Liang;Zhijun Lou;Florian Frugier;Ping Xu;Jeremy D.Murray
CAS-JIC Centre of Excellence for Plant and Microbial Science(CEPAMS),CAS Center for Excellence in Molecular and Plant Sciences,Chinese Academy of Sciences,Shanghai 200032,China Crop Science Centre,Department of Plant Sciences,University of Cambridge,Cambridge CB30LE,UKInstitute of Plant Sciences of Paris-Saclay(IPS2),CNRS,INRAE,Universite´Paris-Saclay,Gif Sur Yvette 91190,France Shanghai Engineering Research Center of Plant Germplasm Resource,College of Life Sciences,Shanghai Normal University,Shanghai 200234,ChinaCAS-JIC Centre of Excellence for Plant and Microbial Science(CEPAMS),CAS Center for Excellence in Molecular and Plant Sciences,Chinese Academy of Sciences,Shanghai 200032,ChinaCAS-JIC Centre of Excellence for Plant and Microbial Science(CEPAMS),CAS Center for Excellence in Molecular and Plant Sciences,Chinese Academy of Sciences,Shanghai 200032,China University of Chinese Academy of Sciences,Beijing 100049,ChinaShanghai Engineering Research Center of Plant Germplasm Resource,College of Life Sciences,Shanghai Normal University,Shanghai 200234,ChinaInstitute of Plant Sciences of Paris-Saclay(IPS2),CNRS,INRAE,Universite´Paris-Saclay,Gif Sur Yvette 91190,FranceCAS-JIC Centre of Excellence for Plant and Microbial Science(CEPAMS),CAS Center for Excellence in Molecular and Plant Sciences,Chinese Academy of Sciences,Shanghai 200032,ChinaCAS-JIC Centre of Excellence for Plant and Microbial Science(CEPAMS),CAS Center for Excellence in Molecular and Plant Sciences,Chinese Academy of Sciences,Shanghai 200032,China Cell and Developmental Biology,John Innes Centre,Norwich NR47UH,UK
农业科技
CRISPR/Cas9Genome editingNodulationSymbiosisGenetic transformationTissue-specific promoter
《aBIOTECH》 2025 (2)
P.346-360,15
funded by the National Natural Science Foundation of China(32200208 to J-PG,and 32170250 to JDM)J-PG is supported by a grant to the University of Cambridge by the Bill&Melinda Gates Foundation and the UK Foreign,Commonwealth and Development Office(OPP1028264)known as the Enabling Nutrient Symbioses in Agriculture(ENSA)projectYS is supported by the China Scholarship Council(202208310095).
评论