Recent advances in scalable exosome production:Challenges and innovationsOA
Exosomes,nanosized extracellular vesicles(30-150 nm),play a crucial role in intercellular communication and are promising biomarkers and therapeutic agents in oncology,neurodegenerative disorders,and immunotherapy.However,their widespread clinical adoption is constrained by challenges in scalable production,efficient purification,and regulatory standardization.This review critically evaluates recent advancements in exosome bioprocessing,including cell source optimization,culture refinement,and next-generation isolation technologies such as microfluidic microarrays and EXODUS systems.Additionally,we address the limitations of current exosome standardization efforts and propose harmonized protocols to enhance reproducibility.Future research should focus on integrating scalable bioreactor-based systems and artificial intelligence-driven quality control frameworks to accelerate exosome applications in precision medicine and regenerative therapy.
Hanfei Chen;Qingfeng Li
Department of Plastic and Reconstructive Surgery,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,200011,ChinaDepartment of Plastic and Reconstructive Surgery,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,200011,China
医药卫生
ExosomeProductionScale-up productionIsolation and purificationStandardization
《Chinese Journal of Plastic and Reconstructive Surgery》 2025 (3)
P.149-163,15
supported by the Shanghai Clinical Research Center of Plastic and Reconstructive Surgery,funded by the Science and Technology Commission of Shanghai Municipality(grant no.22MC1940300)the Shanghai Key Research Center—Shanghai Research Center for Plastic Surgery(grant no.2023ZZ02023).
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