Investigating the vasodilatory effects of baicalin and geniposide on cerebral basal arteries via the TRPV4 channel: Insights into the anti-cerebral ischemic mechanismOA
Background:Baicalin(BC)and geniposide(GD)are effective components of natural remedies,and studies have shown that they protect against cerebral ischemic stroke(CIS).Transient receptor potential vanilloid 4(TRPV4)is a calcium-permeable channel that plays important roles in vascular function and vasodilation.However,no studies are available on the effect of BC/GD on the TRPV4 channel and rat cerebral basilar artery(CBA).This study examined the effect of the combination of BC/GD(7:3)on cerebral vascular function after CIS.Methods:We used western blotting to determine TRPV4 protein levels and live cell fluorescence Ca 2+imaging and patch clamp to determine how BC/GD activates TRPV4 channels.Isolated vessel experiments were used to observe the dilatory effects of BC/GD on CBA under different conditions.Laser Doppler imaging was used to measure cerebral blood flow in rats.Triphenyl tetrazolium chloride and Nissl stainings were used to determine the infarct area in the rat brain and neuronal damage,respectively.Results:BC/GD significantly boosted TRPV4 protein levels in vascular smooth muscle cells(VSMCs)during oxygen-glucose deprivation and increased[Ca 2+]i in TRPV4-HEK 293 cells and VSMCs.This effect was not observed in vector-HEK 293 cells.In patch clamp experiments,BC/GD increased Ca 2+currents in TRPV4-HEK 293 cells,whereas no significant changes were observed in vector-HEK 293 cells.BC/GD dilated CBA contractions induced by U46619 and KCl,with a concentration-dependent increase of the dilatory effect.In the middle cerebral artery occlusion model,cerebral blood flow in the ischemic side significantly decreased,whereas BC/GD intervention significantly increased cerebral blood perfusion in the ischemic side,reduced the infarct area,and improved neurological function scores and neuronal damage.Conclusion:BC/GD activates the TRPV4 channel,leading to Ca ^(2+) influx,which in turn activates the intermediate conductance calcium-activated potassium channels channel to regulate vasodilation in vascular smooth muscle.
Xue-Wei Zhou;An-Dong Zhao;Jia-Hao Zhang;Tian Hu;Chuan Wang;Ji-Ping Liu;Bin Wang
Pharmacology of Chinese Medicine,Shaanxi University of Chinese Medicine,Xianyang 712000,China Key Laboratory of Pharmacodynamics and Material Basis of Chinese Medicine of Shaanxi Administration of Traditional Chinese Medicine,Xianyang 712046,China Engineering Research Center of Brain Health Industry of Chinese Medicine,Universities of Shaanxi Province,Xianyang 712046,ChinaPharmacology of Chinese Medicine,Shaanxi University of Chinese Medicine,Xianyang 712000,ChinaPharmacology of Chinese Medicine,Shaanxi University of Chinese Medicine,Xianyang 712000,China Key Laboratory of Pharmacodynamics and Material Basis of Chinese Medicine of Shaanxi Administration of Traditional Chinese Medicine,Xianyang 712046,China Engineering Research Center of Brain Health Industry of Chinese Medicine,Universities of Shaanxi Province,Xianyang 712046,ChinaPharmacology of Chinese Medicine,Shaanxi University of Chinese Medicine,Xianyang 712000,China Key Laboratory of Pharmacodynamics and Material Basis of Chinese Medicine of Shaanxi Administration of Traditional Chinese Medicine,Xianyang 712046,China Engineering Research Center of Brain Health Industry of Chinese Medicine,Universities of Shaanxi Province,Xianyang 712046,ChinaPharmacology of Chinese Medicine,Shaanxi University of Chinese Medicine,Xianyang 712000,China Key Laboratory of Pharmacodynamics and Material Basis of Chinese Medicine of Shaanxi Administration of Traditional Chinese Medicine,Xianyang 712046,China Engineering Research Center of Brain Health Industry of Chinese Medicine,Universities of Shaanxi Province,Xianyang 712046,ChinaPharmacology of Chinese Medicine,Shaanxi University of Chinese Medicine,Xianyang 712000,China Key Laboratory of Pharmacodynamics and Material Basis of Chinese Medicine of Shaanxi Administration of Traditional Chinese Medicine,Xianyang 712046,China Engineering Research Center of Brain Health Industry of Chinese Medicine,Universities of Shaanxi Province,Xianyang 712046,ChinaPharmacology of Chinese Medicine,Shaanxi University of Chinese Medicine,Xianyang 712000,China Key Laboratory of Pharmacodynamics and Material Basis of Chinese Medicine of Shaanxi Administration of Traditional Chinese Medicine,Xianyang 712046,China Engineering Research Center of Brain Health Industry of Chinese Medicine,Universities of Shaanxi Province,Xianyang 712046,China
医药卫生
baicalingeniposideTRPV4cerebral basilar arterycerebral ischemic stroke
《Traditional Medicine Research》 2026 (2)
P.30-38,9
supported by the Chinese Medicine"Dual Chain Integration"Young and Middle-aged Scientific Research and Innovation Teams(No.2022-SLRH-YQ-006)the Key R&D Programme Projects of Xianyang Municipality(No.L2023-ZDYF-SF-014)the Shaanxi University of Traditional Chinese Medicine Science,Education and Research Collaborative Educational Achievement Transformation Project(No.2024KC03)the open research topic from the Key Laboratory of Neurological Diseases in Traditional Chinese Medicine,Shaanxi Province(No.KF202315).
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