Microwave-assisted vs.conventional hydrodistillation of Corchorus olitorius:a chemical and enzyme inhibition studyOA
Background:Corchorus olitorius L.(Malvaceae)is a green leafy vegetable widely consumed in the Middle East and valued for its rich nutritional content.Its essential oils,an important class of secondary metabolites,are of growing interest for potential use in cosmetics and fragrance industries due to their bioactive properties.Methods:Leaves of C.olitorius cultivated in Egypt were extracted using microwave-assisted hydrodistillation(MAHD)and conventional hydrodistillation(HD).The oils were analyzed by gas chromatography-mass spectrometry(GC-MS)to characterize their chemical profiles.In vitro assays were conducted to evaluate their anti-elastase and anti-collagenase activities,and in silico studies were performed to predict the pharmacokinetic and pharmacodynamic properties of major constituents.Results:GC-MS analysis showed that aldehydes,alkanes,fatty alcohols,fatty acids,and their derivatives were the predominant compound classes in both oils.Notably,oxygenated diterpenes(11.22%)were present exclusively in the MAHD oil,while triterpenoids(16.37%)were found only in the HD oil.The MAHD oil demonstrated stronger In vitro anti-elastase(IC_(50)=42.5μg/mL)and anti-collagenase(IC_(50)=131.5μg/mL)activities compared to the HD oil(IC_(50)=66.7 and 206.8μg/mL,respectively),reflecting an approximate 36.3%improvement in elastase inhibition and 36.4%improvement in collagenase inhibition.In silico docking indicated that the triterpenoidβ-amyrin acetate showed the highest predicted binding affinity for porcine pancreatic elastase(PDB ID:6QEO,ΔG=−8.1 kcal/mol)and collagenase(PDB ID:456C,ΔG=−9.1 kcal/mol)among the major compounds analyzed.Conclusion:These findings demonstrate that MAHD is a greener and more efficient extraction method,yielding oil with enhanced enzyme inhibitory activity compared to conventional HD.The promising anti-elastase and anti-collagenase properties suggest that C.olitorius MAHD oil could serve as a potential candidate for anti-aging cosmetic formulations,following further validation.
Salma Sameh;Ahmed M Elissawy;Eman Al-Sayed;Rola M Labib;Fang-Rong Chang;Abdel Nasser B Singab
Faculty of Pharmacy,Department of Pharmacognosy,Ain-Shams University,Cairo 11566,EgyptFaculty of Pharmacy,Department of Pharmacognosy,Ain-Shams University,Cairo 11566,Egypt Center of Drug Discovery Research&Development,Faculty of Pharmacy,Ain-Shams University,Cairo 11566,EgyptFaculty of Pharmacy,Department of Pharmacognosy,Ain-Shams University,Cairo 11566,EgyptFaculty of Pharmacy,Department of Pharmacognosy,Ain-Shams University,Cairo 11566,EgyptGraduate Institute of Natural Products,College of Pharmacy,Kaohsiung Medical University,Kaohsiung 80708,China Taiwan Department of Medical Research,Kaohsiung Medical University Hospital,Kaohsiung Medical University,Kaohsiung 80708,China Taiwan Drug Development and Value Creation Research Center,Kaohsiung Medical University,Kaohsiung 80708,China TaiwanFaculty of Pharmacy,Department of Pharmacognosy,Ain-Shams University,Cairo 11566,Egypt Center of Drug Discovery Research&Development,Faculty of Pharmacy,Ain-Shams University,Cairo 11566,Egypt
医药卫生
Corchorus olitoriusessential oilsmicrowave-assisted hydrodistillationGas chromatographyenzymecosmetic
《Traditional Medicine Research》 2026 (1)
P.60-74,15
Egyptian Science and Technology and Innovation Fund(STIFA)for the support of the study through grant No.46667 entitled“Sustainability of Lab Capacities of the Center of Drug Discovery Research and Development”.
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