SAC3D1在肝细胞癌中的高表达及其对预后和药敏性的影响OA

Objective To investigate the expression level of SAC3 domain-containing protein 1(SAC3D1)in hepatocellular carcinoma(HCC)and its relationship with prognosis and drug sensitivity.Methods Immunohistochemistry was used to evaluate SAC3D1 expression in HCC and its correlation with clinical parameters.Single-cell RNA sequencing(scRNA-seq)was employed to analyze SAC3D1 expression at the single-cell level in HCC.HCC data from GEO,TCGA,and other databases were integrated to assess SAC3D1 mRNA expression.Univariate Cox analysis and Kaplan-Meier survival analysis were conducted to evaluate the impact of SAC3D1 on HCC prognosis.Drug sensitivity analysis explored the correlation between SAC3D1 and anti-HCC drugs.Enrichment analysis was performed to identify potential pathways through which SAC3D1 promotes HCC progression.Results SAC3D1 protein expression was significantly higher in HCC tissues than in normal liver tissues and positively correlated with age,T stage,and clinical stage(P<0.05).scRNA-seq revealed elevated SAC3D1 expression in epithelial cells.SAC3D1 mRNA was upregulated in HCC(standardized mean difference=1.30,area under the curve=0.90),and its high expression was associated with poor prognosis(hazard ratio=2.17,95%CI=1.42-3.34,P<0.05).Drug sensitivity analysis indicated that SAC3D1 expression correlated with the half-maximal inhibitory concentration of anti-HCC drugs(|R|>0.3,P<0.05).SAC3D1-related genes were enriched in cell cycle-related pathways.Conclusion SAC3D1 may promote HCC progression and drug resistance by regulating cell cycle pathways.