糖尿病患者与健康人A-PRF中成骨与炎症因子的对比研究OA

Objective To compare the release kinetics of key osteogenic growth factors and inflammatory cytokines in advanced platelet-rich fibrin(A-PRF)between patients with diabetes mellitus(DM)and healthy populations.Methods Participants meeting the inclusion and exclusion criteria were assigned to either the DM group(n=8)or the healthy control group(n=8).Venous blood was centrifuged to prepare A-PRF membranes,and eluates were collected at 15 min,60 min,8 h,1 d,3 d,10 d,14 d,and 21 d post-incubation.The concentrations of platelet-derived growth factor-BB(PDGF-BB),vascular endothelial growth factor(VEGF),transforming growth factor-beta(TGF-β),epidermal growth factor(EGF),tumor necrosis factor-alpha(TNF-α),and interleukin-6(IL-6)were quantified using enzyme-linked immunosorbent assay(ELISA)and analyzed comparatively.Results(1)Cumulative PDGF-BB release from A-PRF in the DM group was consistently lower than that in healthy controls,with statistically significant differences observed from 15 min onward(P<0.01).(2)VEGF release from A-PRF was significantly reduced in the DM group from day 1 to day 21 compared to controls(P<0.01).(3)No statistically significant difference in cumulative EGF release was observed between the two groups(P>0.05).(4)Cumulative TGF-β release from A-PRF was significantly lower in the DM group from day 1 onward(P<0.05).(5)TNF-α release from A-PRF was significantly higher in the DM group than in healthy controls from 8 h onward(P<0.05).(6)No statistically significant difference in cumulative IL-6 release was observed between the two groups(P>0.05).Conclusion Compared to healthy individuals,DM patients exhibited significantly lower cumulative release of osteogenic growth factors(PDGF-BB,VEGF,TGF-β)from A-PRF and elevated levels of the inflammatory cytokine TNF-α.No significant differences were observed in EGF and IL-6 release.